Nanoparticles (NPs) are extensively being used in state-of-the-art nano-based therapies, modern electronics, and consumer products, so can be released into the environment with enhancement interaction with humans. Hence, the exposures to these multifunctional NPs lead to changes in protein structure and functionality, raising serious health issues. This study thoroughly investigated the interaction and adsorption of catalase (CAT) with HfO 2 -NPs by circular dichroism (CD), Fourier transform infrared (FTIR), absorption, and fluorescence spectroscopic techniques. The results indicate that HfO 2 NPs cause fluorescence quenching in CAT by a static quenching mechanism. The negative values of Vant Hoff thermodynamic expressions (Δ H o , Δ S o , and Δ G o ) corroborate the spontaneity and exothermic nature of static quenching driven by van der Waals forces and hydrogen bonding. Also, FTIR, UV...

中文翻译：

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在过氧化氢酶的生物生理微环境中解释氧化oxide纳米粒子的扰动机理

纳米颗粒（NPs）被广泛用于先进的基于纳米的疗法，现代电子产品和消费产品中，因此可以通过与人类的增强互动而释放到环境中。因此，暴露于这些多功能NP会导致蛋白质结构和功能的改变，从而引发严重的健康问题。这项研究通过圆二色性（CD），傅立叶变换红外（FTIR），吸收和荧光光谱技术彻底研究了过氧化氢酶（CAT）与HfO 2 -NP的相互作用和吸附。结果表明，HfO 2 NPs通过静态猝灭机制引起CAT中的荧光猝灭。Vant Hoff热力学表达式的负值（ΔH o，ΔS o，和ΔG o）证实了范德华力和氢键驱动的静态猝灭的自发性和放热性。此外，FTIR，UV ...