This paper describes the experimental monitoring of pancreatic oxidative metabolism in laboratory mice that combines the methods of fluorescence and diffuse reflectance spectroscopy and laser speckle contrast imaging with a pancreatic ischemia model. The combined measurements show a close dependence of tissue metabolism on blood and oxygen supply. We show that deactivation of complex I and complex II occurs in mouse pancreatic tissue during prolonged hypoxia. We conclude that complex I can potentially undergo more intensive deactivation when oxygen is lacking than complex II. We have demonstrated that the methods described can be applied in minimally invasive surgery of the pancreas to assess its viability.

中文翻译：

---

使用多峰光谱技术在小鼠胰腺缺血建模中监测氧化代谢

本文介绍了对实验小鼠胰腺氧化代谢的实验监测，该实验将荧光和漫反射光谱法以及激光斑点对比成像与胰腺缺血模型相结合。组合的测量结果显示组织代谢与血液和氧气供应密切相关。我们显示，在长时间缺氧期间，小鼠胰腺组织中发生了复合物I和复合物II的失活。我们得出的结论是，当缺乏氧气时，复合物I可能比复合物II经历更强烈的失活。我们已经证明，所描述的方法可以应用于胰腺的微创手术以评估其生存能力。