This work reports the isolation of the protein from the flour of an underutilised agro waste, a de-oiled cake of Madhuca latifolia using the bis (2-ethylehexyl) sodium sulfosuccinate salt reverse micelle and the characterisation of the protein through various techniques. The experimental conditions for the extraction were optimised using Box–Behnken design. The highest yield of the protein was achieved when the extraction parameters, i.e. KCl concentration, KCl amount, and pH of the medium, were 0.5 M, 1.25 ml, and 9.02, respectively. The experimental yield (75.56%) obtained under the optimised conditions matched extremely well with the predicted yield (75.19%). The analysis of the biochemical composition envisaged the occurrence of 2S albumin, 7S globulin, and 11S globulin as the major components in the protein. The X-ray diffraction pattern supported the β-sheets structure of the protein. The imaging of the protein through a scanning electron microscope revealed the shape and surface of the protein to be spherical and smooth, respectively. Thus, the protein isolate of the de-oiled cake flour of Madhuca latifolia could be utilised towards food product development and relevant fields.

中文翻译：

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马杜卡去油饼粉中蛋白质的分离、工艺优化和表征

这项工作报告了从未充分利用的农业废物的面粉中分离出蛋白质，这是一种脱油蛋糕。阔叶马杜卡使用双（2-乙基己基）磺基琥珀酸钠盐反胶束并通过各种技术表征蛋白质。使用 Box-Behnken 设计优化了提取的实验条件。当提取参数，即KCl浓度、KCl量和培养基的pH值分别为0.5 M、1.25 ml和9.02时，蛋白质的产量最高。在优化条件下获得的实验产率（75.56%）与预测产率（75.19%）非常吻合。生化成分分析设想存在 2S 白蛋白、7S 球蛋白和 11S 球蛋白作为蛋白质中的主要成分。X 射线衍射图支持蛋白质的 β 折叠结构。通过扫描电子显微镜对蛋白质的成像显示蛋白质的形状和表面分别为球形和光滑。因此，脱油蛋糕粉的分离蛋白阔叶马杜卡可用于食品开发和相关领域。