This is the first study to report the green synthesis of Lobelia trigona Roxb- mediated silver nanoparticles (LTAgNPs). The optical and structural properties of the synthesised LTAgNPs were analysed using ultraviolet–visible spectroscopy, scanning electron microscopy, Fourier transform infrared, dynamic light scattering and energy dispersive X-ray. LTAgNps were evaluated for their anti-bacterial and anti-fungal properties against 18 pathogens and exhibited significant inhibition against all the strains tested. LTAgNPs had potential scavenging effects on the DPPH, ^• OH, O ₂ ^•− free radical scavenging assays and reducing power assay. LTAgNps possess strong anti-cancer activity against five human cancer cell lines (A549, MCF-7, MDA-MB-231, HeLa and KB) in a dose-dependent manner. The antiproliferative, anti-inflammatory and genotoxicity effects of LTAgNPs were further confirmed by the lactate dehydrogenase release assay, nitric oxide inhibitory assay and comet assay. Furthermore, the incision, excision and burn wound-healing activity of formulated LTAgNPs ointment was assessed in rats. All the wounds had significant healing in groups treated with LTAgNPs ointment compared to the groups treated with the commonly prescribed ointment (Silverex ^TM ). This study shows and suggests that the previously unreported LTAgNPs could be used as a nanomedicine with significant biological applications.

中文翻译：

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具有增强生物学应用的基于半边莲 Roxb 的纳米药物：体外和体内方法

这是第一个报告绿色合成的研究半边莲 介导的银纳米粒子（LTAgNPs）。使用紫外-可见光谱、扫描电子显微镜、傅里叶变换红外、动态光散射和能量色散 X 射线分析合成的 LTAgNPs 的光学和结构特性。评估了 LTAgNps 对 18 种病原体的抗菌和抗真菌特性，并对所有测试的菌株表现出显着的抑制作用。LTAgNPs 对 DPPH 有潜在的清除作用，^• OH, O ₂ ^•−自由基清除试验和还原能力试验。LTAgNps 以剂量依赖性方式对五种人类癌细胞系（A549、MCF-7、MDA-MB-231、HeLa 和 KB）具有很强的抗癌活性。乳酸脱氢酶释放试验、一氧化氮抑制试验和彗星试验进一步证实了 LTAgNPs 的抗增殖、抗炎和遗传毒性作用。此外，在大鼠中评估了配制的 LTAgNPs 软膏的切口、切除和烧伤创面愈合活性。与使用常用处方药膏 (Silverex ^TM ）。这项研究表明并表明以前未报道的 LTAgNPs 可用作具有重要生物学应用的纳米药物。