当前位置：
X-MOL 学术
›
J. Inflammation Res.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
HPV-16 E2/E6 and POU5F1B as Biomarkers to Determine Cervical High-Grade Squamous Lesions and More
Journal of Inflammation Research ( IF 4.2 ) Pub Date : 2020-10-29 , DOI: 10.2147/jir.s278911 Lihua Chen 1, 2 , Binhua Dong 2, 3 , Hangjing Gao 2, 3 , Huifeng Xue 4 , Diling Pan 5 , Pengming Sun 1, 2, 3
Journal of Inflammation Research ( IF 4.2 ) Pub Date : 2020-10-29 , DOI: 10.2147/jir.s278911 Lihua Chen 1, 2 , Binhua Dong 2, 3 , Hangjing Gao 2, 3 , Huifeng Xue 4 , Diling Pan 5 , Pengming Sun 1, 2, 3
Affiliation
Purpose: Human papillomavirus-16 (HPV-16) is the most carcinogenic HPV genotype. This study aimed to evaluate the clinical value of POU5F1B and HPV-16-E2/E6 by cervical cytology specimens to predict the cervical intraepithelial neoplasia two grade and more (CIN2+).
Methods: Finally, 248 patients with HPV-16 single infection were enrolled. Using cytology specimen by real-time quantitative PCR (qPCR), POU5F1B mRNA and HPV-16-E2/E6 were detected. The relationship of POU5F1B, HPV-16-E2/E6 and CIN2+ were analyzed, and the optimal cut-off values of POU5F1B and HPV-16-E2/E6 to predict CIN2+ were calculated.
Results: The mean HPV-16-E2/E6 decreased significantly with cervical lesions development, especially compared with CIN2+ (p< 0.05). And the POU5F1B demonstrated higher expression in CIN2+ than that of normal cervical tissue and CIN1 (p< 0.05). What is more, POU5F1B was negatively correlated with HPV-16-E2/E6. It demonstrated that the area under the receiver operating characteristic curve (AUC) for POU5F1B (0.9058) was higher than that for HPV-16-E2/E6 (0.8983), and the sensitivity and specificity of POU5F1B in the diagnosis of CIN2+ were higher than HPV-E2/E6. Furthermore, it demonstrated that the POU5F1B had the highest odds ratio (OR= 16.84; 95% CI (8.00– 35.46)) for the detection of CIN 2+.
Conclusion: HPV-16-E2/E6≤ 0.6471 or POU5F1B≥ 1.0310 in cervical exfoliated cells can be used as a reliable predictor of CIN2+. POU5F1B can be used as a new auxiliary biomarker to determine the HPV infection status and a reliable predictor of CIN2+. The expression of POU5F1B≥ 1.0310 had the highest OR for the detection of CIN2+.
Keywords: cervical intraepithelial neoplasia, human papillomavirus type 16, POU5F1B, HPV integration, HPV-E2/E6
中文翻译：
HPV-16 E2/E6 和 POU5F1B 作为生物标志物确定宫颈高度鳞状病变等
目的：人乳头瘤病毒16（HPV-16）是最致癌的HPV基因型。本研究旨在通过宫颈细胞学标本评估POU5F1B和HPV-16-E2/E6在预测宫颈上皮内瘤变2级及以上（CIN2+）的临床价值。
方法：最终纳入248例HPV-16单一感染患者。使用细胞学标本通过实时定量PCR（qPCR）检测POU5F1B mRNA和HPV-16-E2/E6。分析POU5F1B、HPV-16-E2/E6和CIN2+的关系，计算POU5F1B和HPV-16-E2/E6预测CIN2+的最佳截断值。
结果：随着宫颈病变的发展，平均 HPV-16-E2/E6 显着降低，特别是与 CIN2+ 相比（p<0.05）。POU5F1B在CIN2+中的表达高于正常宫颈组织和CIN1（p<0.05）。更重要的是，POU5F1B与HPV-16-E2/E6呈负相关。表明POU5F1B的受试者工作特征曲线下面积（AUC）（0.9058）高于HPV-16-E2/E6（0.8983），POU5F1B诊断CIN2+的敏感性和特异性高于HPV-E2/E6。此外，它表明 POU5F1B 检测 CIN 2+ 的优势比最高（OR= 16.84；95% CI (8.00–35.46)）。
结论：宫颈脱落细胞中HPV-16-E2/E6≤0.6471或POU5F1B≥1.0310可作为CIN2+的可靠预测指标。POU5F1B 可作为一种新的辅助生物标志物来确定 HPV 感染状态和 CIN2+ 的可靠预测因子。POU5F1B≥1.0310的表达对CIN2+的检测具有最高的OR值。
关键词：宫颈上皮内瘤变，人乳头瘤病毒16型，POU5F1B，HPV整合，HPV-E2/E6
更新日期：2020-10-30
Methods: Finally, 248 patients with HPV-16 single infection were enrolled. Using cytology specimen by real-time quantitative PCR (qPCR), POU5F1B mRNA and HPV-16-E2/E6 were detected. The relationship of POU5F1B, HPV-16-E2/E6 and CIN2+ were analyzed, and the optimal cut-off values of POU5F1B and HPV-16-E2/E6 to predict CIN2+ were calculated.
Results: The mean HPV-16-E2/E6 decreased significantly with cervical lesions development, especially compared with CIN2+ (p< 0.05). And the POU5F1B demonstrated higher expression in CIN2+ than that of normal cervical tissue and CIN1 (p< 0.05). What is more, POU5F1B was negatively correlated with HPV-16-E2/E6. It demonstrated that the area under the receiver operating characteristic curve (AUC) for POU5F1B (0.9058) was higher than that for HPV-16-E2/E6 (0.8983), and the sensitivity and specificity of POU5F1B in the diagnosis of CIN2+ were higher than HPV-E2/E6. Furthermore, it demonstrated that the POU5F1B had the highest odds ratio (OR= 16.84; 95% CI (8.00– 35.46)) for the detection of CIN 2+.
Conclusion: HPV-16-E2/E6≤ 0.6471 or POU5F1B≥ 1.0310 in cervical exfoliated cells can be used as a reliable predictor of CIN2+. POU5F1B can be used as a new auxiliary biomarker to determine the HPV infection status and a reliable predictor of CIN2+. The expression of POU5F1B≥ 1.0310 had the highest OR for the detection of CIN2+.
Keywords: cervical intraepithelial neoplasia, human papillomavirus type 16, POU5F1B, HPV integration, HPV-E2/E6
中文翻译：
HPV-16 E2/E6 和 POU5F1B 作为生物标志物确定宫颈高度鳞状病变等
目的：人乳头瘤病毒16（HPV-16）是最致癌的HPV基因型。本研究旨在通过宫颈细胞学标本评估POU5F1B和HPV-16-E2/E6在预测宫颈上皮内瘤变2级及以上（CIN2+）的临床价值。
方法：最终纳入248例HPV-16单一感染患者。使用细胞学标本通过实时定量PCR（qPCR）检测POU5F1B mRNA和HPV-16-E2/E6。分析POU5F1B、HPV-16-E2/E6和CIN2+的关系，计算POU5F1B和HPV-16-E2/E6预测CIN2+的最佳截断值。
结果：随着宫颈病变的发展，平均 HPV-16-E2/E6 显着降低，特别是与 CIN2+ 相比（p<0.05）。POU5F1B在CIN2+中的表达高于正常宫颈组织和CIN1（p<0.05）。更重要的是，POU5F1B与HPV-16-E2/E6呈负相关。表明POU5F1B的受试者工作特征曲线下面积（AUC）（0.9058）高于HPV-16-E2/E6（0.8983），POU5F1B诊断CIN2+的敏感性和特异性高于HPV-E2/E6。此外，它表明 POU5F1B 检测 CIN 2+ 的优势比最高（OR= 16.84；95% CI (8.00–35.46)）。
结论：宫颈脱落细胞中HPV-16-E2/E6≤0.6471或POU5F1B≥1.0310可作为CIN2+的可靠预测指标。POU5F1B 可作为一种新的辅助生物标志物来确定 HPV 感染状态和 CIN2+ 的可靠预测因子。POU5F1B≥1.0310的表达对CIN2+的检测具有最高的OR值。
关键词：宫颈上皮内瘤变，人乳头瘤病毒16型，POU5F1B，HPV整合，HPV-E2/E6
京公网安备 11010802027423号