Herein, the cytotoxicity of a novel zwitterionic sulfobetaine hydrogel system with a nano-clay crosslinker has been investigated. We demonstrate that careful selection of the composition of the system (monomer to Laponite content) allows the material to be formed into controlled shapes using an extrusion based additive manufacturing technique with the ability to tune the mechanical properties of the product. Moreover, the printed structures can support their own weight without requiring curing during printing which enables the use of a printing-then-curing approach. Cell culture experiments were conducted to evaluate the neural cytotoxicity of the developed hydrogel system. Cytotoxicity evaluations were conducted on three different conditions; a control condition, an indirect condition (where the culture medium used had been in contact with the hydrogel to investigate leaching) and a direct condition (cells growing directly on the hydrogel). The result showed no significant difference in cell viability between the different conditions and cells were also found to be growing on the hydrogel surface with extended neurites present.

在此，研究了具有纳米粘土交联剂的新型两性离子磺基甜菜碱水凝胶系统的细胞毒性。我们证明，通过仔细选择系统的成分（单体到合成锂皂石含量），可以使用基于挤出的增材制造技术将材料形成受控形状，并能够调整产品的机械性能。此外，打印的结构可以支撑其自身重量，而无需在打印过程中进行固化，这使得可以使用打印然后固化的方法。进行细胞培养实验以评估所开发的水凝胶系统的神经细胞毒性。在三种不同条件下进行细胞毒性评估；对照条件、间接条件（所使用的培养基与水凝胶接触以研究浸出）和直接条件（细胞直接在水凝胶上生长）。结果显示，不同条件下的细胞活力没有显着差异，并且还发现细胞在水凝胶表面上生长，并存在延伸的神经突。