A modified protocol for the extraction and analysis of cortisol in individual zebrafish, Danio rerio, embryo, and larva samples has been developed and evaluated. Recovery efficiency of the method was high, specifically calculated at 93.8% ± 6.5%. Dilution tests showed high parallelism, while increasing the number of individuals used in each extraction sample resulted in a linear, although slightly underestimated, increase of cortisol yield. Results of cortisol content from 0, 3, and 5 days postfertilization (dpf) fish using the proposed protocol were within the range of most published studies analyzing cortisol in pooled samples of 10–30 individuals. Moreover, 5 dpf larvae had significantly higher cortisol levels than embryos, a pattern commonly observed in literature. Finally, application of an osmotic stress in 5 dpf larvae led to a statistically significant increase in cortisol content.

中文翻译：

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斑马鱼（斑马鱼），单个胚胎和幼虫中皮质醇分析的修改的协议。

修改后的协议，用于提取和分析单个斑马鱼中的皮质醇，Danio rerio，胚胎和幼虫的样品已经开发和评估。该方法的回收率很高，具体计算为93.8％±6.5％。稀释测试显示出高度的平行性，同时增加了每个提取样品中使用的个体数量，导致线性（尽管被低估了）皮质醇的产量增加。使用建议的方案从受精后0、3和5天的鱼中皮质醇含量的结果在大多数已发表的分析10-30个人样本中皮质醇的研究范围内。此外，5 dpf幼虫的皮质醇水平明显高于胚胎，这是文献中常见的模式。最后，在5 dpf幼虫中施加渗透胁迫导致皮质醇含量在统计学上显着增加。