CRISPR-Cas-based transcriptional activators allow genetic engineers to specifically induce expression of one or many target genes in trans. Here we review the many design variations of these versatile tools and compare their effectiveness in different eukaryotic systems. Lastly, we highlight several applications of programmable transcriptional activation to interrogate and engineer complex biological processes.

中文翻译：

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用于在高等真核生物中工程基因表达的 CRISPR-Cas 激活剂

基于 CRISPR-Cas 的转录激活剂允许遗传工程师特异性地诱导一种或多种靶基因的反式表达。在这里，我们回顾了这些多功能工具的许多设计变体，并比较了它们在不同真核系统中的有效性。最后，我们重点介绍了可编程转录激活在询问和设计复杂生物过程中的几种应用。