Oxidative stress can impede maturation of the nucleus and cytoplasm of oocytes during in vitro maturation (IVM). Rhodiola sachalinensis, an herb commonly used in traditional Chinese medicine, conveys antioxidative effects to cryopreserved bovine sperm. Therefore, the aims of this study were to evaluate the effects of different concentrations of R. sachalinensis aqueous extract (RSAE) on IVM and subsequent in vitro embryonic development after parthenogenetic activation (PA), in vitro fertilization (IVF), and somatic cell nuclear transfer (SCNT). The results showed that RSAE supplementation (6 and 60 mg/L) significantly increased intracellular glutathione levels, but had no effect on maturation rates or reactive oxygen species. After in vitro culture, greater blastocyst formation was observed in PA embryos (6 mg/L RSAE), as well as in IVF and SCNT embryos (60 mg/L) matured in RSAE-supplemented IVM media. In conclusion, although there was no significant improvement in the maturation rate, RSAE supplementation conveyed an antioxidative effect during IVM, and improved subsequent embryonic development in vitro. Further studies are needed to explore gene expression pattern in oocytes and embryos treated with RSAE.

中文翻译：

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红景天水提物对猪卵母细胞体外成熟及随后体外胚胎发育的影响

氧化应激可在体外成熟 (IVM)期间阻碍卵母细胞的细胞核和细胞质的成熟。Rhodiola sachalinensis是一种中药常用的草药，对冷冻保存的牛精子具有抗氧化作用。因此，本研究的目的是在体外评估不同浓度的R. sachalinensis水提取物 (RSAE) 对 IVM 和随后在孤雌生殖激活 (PA) 后体外胚胎发育的影响。受精（IVF）和体细胞核移植（SCNT）。结果表明，添加 RSAE（6 和 60 mg/L）可显着增加细胞内谷胱甘肽水平，但对成熟率或活性氧种类没有影响。在体外培养后，在 PA 胚胎 (6 mg/L RSAE) 以及在添加 RSAE 的 IVM 培养基中成熟的 IVF 和 SCNT 胚胎 (60 mg/L) 中观察到更多的囊胚形成。总之，虽然成熟率没有显着改善，但 RSAE 补充剂在 IVM 期间具有抗氧化作用，并改善了随后的体外胚胎发育。需要进一步的研究来探索用 RSAE 处理的卵母细胞和胚胎中的基因表达模式。