In this study, an attempt was made to synthesize silver nanoparticles (Ag-NPs) using Grewia optiva leaf extract and isolated compounds. The bioreductant capacity of Grewia optiva leaf extract for the synthesis of Ag-NPs was assessed using various confirmatory techniques like thermogravimetric analysis (TGA), particle size analysis (PSA), energy-dispersive X-ray (EDX), X-ray diffraction (XRD) analysis, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and UV-Visible spectroscopy. The presence of various bioactive compounds in leaf aqueous extract was confirmed through HPLC analysis, and 8 compounds were identified among the different peaks present in the chromatogram. Biopotencies like antioxidant, antibacterial, and effect on hair growth were determined for extract and NPs. Antioxidant capacities were assessed through standard ABTS and DPPH methods. The antibacterial potential was evaluated in terms of zone of inhibition, minimum bactericidal concentration, and minimum inhibitory concentration of the Ag-NPs and the leaf extract against selected strains of bacteria, whereas the effect on growth of rabbit hair was studied through topical treatment for a specific period of time. Better antibacterial and DPPH and ABTS free radical inhibition was observed for the formulated Ag-NPs as compared to leaf extract. The previously isolated eight compounds from this plant’s chloroform and ethyl acetate extracts were also tested for their bioreductant capacities. Out of them, the highest amount of precipitates was obtained with compound VII ((2,5-dihydroxyphenyl)-3,6,8-trihydroxyl-4H-chromen-4-one). The study implies that the biogenically engineered nanoscale particles could have promising biological activities in comparison to parental extract and they need to be investigated further as potential therapeutic agents to be used as antibacterial and antioxidant agents and for hair growth enhancement.

中文翻译：

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番石榴叶水提取物和分离的化合物作为还原剂绿色合成银纳米粒子及其生物活性

在这项研究中，人们尝试使用黄rew叶提取物和分离出的化合物合成银纳米颗粒（Ag-NPs）。黄花菜的生物还原能力使用多种确认性技术，如热重分析（TGA），粒度分析（PSA），能量色散X射线（EDX），X射线衍射（XRD）分析，扫描电子，评估了用于合成Ag-NPs的叶提取物显微镜（SEM），傅立叶变换红外光谱（FTIR）和紫外可见光谱。通过HPLC分析确认了叶水提取物中各种生物活性化合物的存在，并在色谱图中存在的不同峰中鉴定出8种化合物。确定了提取物和NP的抗氧化，抗菌和对头发生长的影响等生物功效。通过标准ABTS和DPPH方法评估抗氧化能力。根据抑菌圈，最小杀菌浓度，以及最小的Ag-NPs和叶提取物对所选细菌菌株的抑制浓度，而通过局部处理一段时间来研究对兔毛生长的影响。与叶提取物相比，对配制的Ag-NPs观察到更好的抗菌，DPPH和ABTS自由基抑制作用。还测试了从该工厂的氯仿和乙酸乙酯提取物中分离出的八种化合物的生物还原能力。其中，用化合物（（2,5-二羟基苯基）-3）获得的沉淀量最高 与叶提取物相比，对配制的Ag-NPs观察到更好的抗菌，DPPH和ABTS自由基抑制作用。还测试了从该工厂的氯仿和乙酸乙酯提取物中分离出的八种化合物的生物还原能力。其中，用化合物（（2,5-二羟基苯基）-3）获得的沉淀量最高 与叶提取物相比，对配制的Ag-NPs观察到更好的抗菌，DPPH和ABTS自由基抑制作用。还测试了从该工厂的氯仿和乙酸乙酯提取物中分离出的八种化合物的生物还原能力。其中，用化合物（（2,5-二羟基苯基）-3）获得的沉淀量最高， 6 ， 8 -三羟基-4H-苯并吡喃-4- -酮）。该研究表明，与亲本提取物相比，生物工程化的纳米级颗粒可能具有有前途的生物活性，因此有必要对其进行进一步研究，以作为潜在的治疗剂用作抗菌剂和抗氧化剂并促进毛发生长。