A non-invasive and more sensitive method for detection of HE4 is very important for the early screening and detection of ovarian carcinoma. In this study, we improved our previous localized surface plasmon resonance (LSPR) biosensor for detection of HE4 in urine to overcome disadvantages of conventional methods. Protein G directional fixed method was firstly used for LSPR biosensor to improved sensitivity, and standard HE4 and clinical samples were detected separately using this new biosensor. Compared to our previous LSPR biosensor, this new sensor was more sensitive, with other advantages as before. Under optimum conditions, this new biosensor could display a detection limit of 1 pM and wide dynamic range of 1 pM to 10,000 pM. This new biosensor was effective for detection of HE4 in urine of early ovarian cancer patients, without label and purification. To the best of our knowledge, this is first work to investigate LSPR biosensor for detection of tumor marker in urine, with great advantages and clinical application potentials.

中文翻译：

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基于局部表面等离子体共振和G蛋白定向固定的新型尿液HE4检测无标记生物传感器

一种非侵入性且更灵敏的HE4检测方法对于卵巢癌的早期筛查和检测非常重要。在这项研究中，我们改进了先前的局部表面等离振子共振（LSPR）生物传感器，用于检测尿液中的HE4，以克服传统方法的缺点。首次将蛋白G方向固定方法用于LSPR生物传感器，以提高灵敏度，并使用此新型生物传感器分别检测标准HE4和临床样品。与我们以前的LSPR生物传感器相比，这种新型传感器更加灵敏，并具有其他优势。在最佳条件下，这种新型生物传感器的检测限为1 pM，动态范围为1 pM至10,000 pM。这种新型生物传感器可有效检测早期卵巢癌患者尿液中的HE4，没有标签和纯化。据我们所知，这是研究LSPR生物传感器用于尿液中肿瘤标志物检测的第一项工作，具有很大的优势和临床应用潜力。