In order to improve the practicality of the rapid biochemical oxygen demand (BOD) method, a highly sensitive rapid detection method for BOD that is based on establishing the correlation between current and dissolved oxygen (DO) was developed. In this experiment, Bacillus subtilis was used as the test microorganism, and the embedding method was used to achieve quantitative fixation of microorganisms, which could increase the content of microorganisms and prolong the service life of the biological element. The conductivity (COND) probe is used as a sensing element, so that the testing value can be read every second. In the program, the moving average method is used to process the collected data so that the value can be read every minute. National standard samples were detected to test the accuracy and stability of the method. The results showed that relative error and analytical standard deviations were less than 5%. Different polluted water was tested to evaluate its application range. The results showed that relative error was less than 5%. The results of the method are consistent with the results of the wastewater sample obtained by the BOD₅ standard method. The proposed rapid BOD current sensing biosensor method should be promising in practical application of wastewater monitoring.

中文翻译：

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一种用于 BOD 快速测量的电流传感生物传感器

为了提高快速生化需氧量（BOD）方法的实用性，开发了一种基于建立电流与溶解氧（DO）相关性的高灵敏度BOD快速检测方法。本实验中，枯草芽孢杆菌以微生物为试验微生物，采用包埋法实现微生物的定量固定，可增加微生物的含量，延长生物元件的使用寿命。电导（COND）探头作为传感元件，可以每秒读取测试值。程序中采用移动平均法对采集到的数据进行处理，实现每分钟读取一次数值。检测国家标准样品，检验方法的准确性和稳定性。结果表明，相对误差和分析标准偏差均小于5%。测试了不同的污染水，以评估其应用范围。结果表明，相对误差小于5%。₅标准方法。所提出的快速 BOD 电流传感生物传感器方法在废水监测的实际应用中应该是有前途的。