A thermal cycling method, whereby capillary tubes holding polymerase chain reactions are subjected to programmed tilt displacements so that they are moved using gravity over three spatial regions (I, II, and III) kept at different constant temperatures to facilitate deoxyribonucleic acid (DNA) denaturation, annealing, and extension, is described. At tilt speeds in excess of 0.2 rad/s, the standard deviation of static coefficient of friction values was below 0.03, indicating in sync movement of multiple capillary tubes over the holding platform. The travel time during the acceleration phase and under constant velocity between adjacent regions (I to II and II to III) and distant regions (III to I) was 0.03 s and 0.31 s, respectively. The deviations in temperature did not exceed 0.05 °C from the average at the prescribed denaturing, annealing, and extension temperatures applied. DNA amplification was determined by optical readings, the fluorescence signal was found to increase twofold after 30 thermal cycles, and 1.16 × 106 DNA copies/μl could be detected. The approach also overcomes problems associated with thermal inertia, sample adhesion, sample blockage, and handling of the reaction vessels encountered in the other thermal cycling schemes used.

中文翻译：

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使用毛细管的程序倾斜位移进行聚合酶链反应热循环

一种热循环方法，其中保持聚合酶链反应的毛细管受到程序化的倾斜位移，以便它们利用重力在保持不同恒温的三个空间区域（I、II 和 III）上移动，以促进脱氧核糖核酸 (DNA) 变性，退火和延伸，进行了描述。在超过 0.2 弧度/秒的倾斜速度下，静摩擦系数值的标准偏差低于 0.03，表明多个毛细管在保持平台上同步运动。加速阶段和等速下相邻区域（I至II和II至III）与远区域（III至I）之间的旅行时间分别为0.03 s和0.31 s。在规定的变性、退火、和延伸温度。通过光学读数确定 DNA 扩增，发现在 30 个热循环后荧光信号增加了两倍，并且可以检测到 1.16 × 106 DNA 拷贝/μl。该方法还克服了在使用的其他热循环方案中遇到的与热惯性、样品粘附、样品堵塞和反应容器处理相关的问题。