Breeding for resistance to biotic stress and higher yield is a continuous process. Thus, the identification of desirable parents with good combining ability and nature of gene action for the target trait is of utmost importance. Hence, in this present investigation, 10 lines and three testers of Okra were crossed in line × tester mating design to generate 30 testcross progenies and their evaluation along with parents and check in a randomized complete block design with three replications. To depict the true picture of genetic variation among the parental genotypes, molecular diversity analysis was also carried out using genomic-simple sequence repeats before crossing to ascertain that sufficient variability is present among the parents. The molecular analysis grouped the parental genotypes into four clusters (I–IV). The analysis of variance revealed that all the treatments were significant for most of the traits. The combining ability analysis suggested Pusa A-4 as the best general combiner for earliness, Pusa Bhindi-5 for high yield, and DOV-92 for fruit length, plant height, yield per plant, and coefficient of infection for Yellow Vein Mosaic Virus Disease resistance. Similarly, the specific combining ability analysis suggested that the cross combinations DOV-92 × Pusa Bhindi-5 followed by DOV-92 × Pusa A-4 and DOV-92 × Pusa Sawani exhibit high economic heterosis for yield per plant as well as for disease resistance. Finally, estimation of the degree of dominance and predictability ratio was also worked out which indicated the prevalence of non-additive gene action for most of the traits pointing towards sufficient scope for heterosis breeding in Okra.

中文翻译：

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确定秋葵 (Abelmoschus esculentus (L.) Monech.) 产量贡献性状和黄脉花叶病毒 (YVMV) 抗性的遗传结合能力、杂种优势潜力和基因作用

抗生物胁迫和更高产量的育种是一个持续的过程。因此，为目标性状鉴定具有良好结合能力和基因作用性质的理想亲本至关重要。因此，在本次调查中，10 个品系和 3 个秋葵测试者在行 × 测试者交配设计中交叉，以产生 30 个测试交叉后代及其与亲本的评估，并检查具有三个重复的随机完整区组设计。为了描绘亲本基因型之间遗传变异的真实情况，还使用杂交前的基因组简单序列重复进行分子多样性分析，以确定亲本之间存在足够的变异性。分子分析将亲本基因型分为四个簇（I-IV）。方差分析表明，所有处理对大多数性状均具有显着性。配合力分析表明，Pusa A-4 是最好的早熟通用配合剂，Pusa Bhindi-5 是高产的，DOV-92 是果实长度、株高、单株产量和黄脉花叶病毒病感染系数的最佳组合。反抗。同样，特定配合力分析表明，杂交组合 DOV-92 × Pusa Bhindi-5，其次是 DOV-92 × Pusa A-4 和 DOV-92 × Pusa Sawani，在单株产量和病害方面表现出较高的经济杂种优势反抗。最后，还估算了优势程度和可预测性比率，这表明大多数性状的非加性基因作用普遍存在，表明秋葵有足够的杂种优势育种空间。