Plant disease is one of the most important causes of crop losses worldwide. The effective control of plant disease is related to food security. Sclerotinia stem rot (SSR) caused by Sclerotinia sclerotiorum leads to serious yield losses in rapeseed (Brassica napus) production. Hypovirulent strain DT-8 of S. sclerotiorum, infected with Sclerotinia sclerotiorum hypovirulence-associated DNA virus 1 (SsHADV-1), has the potential to control SSR. In this study, we found rapeseed bio-priming with strain DT-8 could significantly decrease the disease severity of SSR and increase yield in the field. After bio-priming, strain DT-8 could be detected on the aerial part of the rapeseed plant. By 16S rRNA gene and internal transcribed spacer (ITS) sequencing technique, the microbiome on different parts of the SSR lesion on bioprimed and non-bioprimed rapeseed stem was determined. The results indicated that SSR and bio-priming treatment could influence the structure and composition of fungal and bacterial communities. Bio-priming treatment could reduce the total abundance of possible plant pathogens and enhance the connectivity and robustness of the interaction network at the genus level. This might be one of the mechanisms that rapeseed bioprimed with strain DT-8 had excellent tolerance on SSR. It might be another possible mechanism of biocontrol and will provide a theoretical guide for agricultural practical production.

植物病害是全世界农作物损失的最重要原因之一。植物病害的有效防治关系到粮食安全。核盘菌茎腐病（SSR）是由核盘菌引起的，导致油菜（甘蓝型油菜）生产严重减产。感染了核盘菌低毒力相关DNA病毒1（SsHADV-1）的核盘菌低毒力菌株DT-8，具有控制SSR的潜力。在这项研究中，我们发现使用菌株 DT-8 进行油菜生物引发可以显着降低 SSR 疾病的严重程度并提高田间产量。生物引发后，可以在油菜植株的地上部分检测到菌株DT-8。通过16S rRNA基因和内转录间隔区（ITS）测序技术，测定了生物底漆和非生物底漆油菜茎上SSR病变不同部位的微生物组。结果表明，SSR 和生物引发处理可以影响真菌和细菌群落的结构和组成。生物引发处理可以减少可能的植物病原体的总丰度，并增强属水平上相互作用网络的连通性和稳健性。这可能是DT-8菌株生物底漆处理的油菜籽对SSR具有优异耐受性的机制之一。这可能是另一种可能的生物防治机制，将为农业实际生产提供理论指导。