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Determination of Phytochemical Content by Chromatographic Methods and Antioxidant Capacity in Methanolic Extract of Jujube (Zizyphus jujuba Mill.) and Oleaster (Elaeagnus angustifolia L.)
International Journal of Fruit Science ( IF 2.4 ) Pub Date : 2020-09-21 , DOI: 10.1080/15538362.2020.1834900
Hafize Dilek Tepe 1 , Fatma Doyuk 1
Affiliation  

ABSTRACT In this study, the antioxidant effects, protein contents and phytochemical structure content of jujube, oleaster fruits and their seeds were investigated. Methanol extraction of fruits and seeds was prepared and volatile organic components were examined by GC-MS and phenolic components were examined by LC-MS/MS. Antioxidant activity was determined by DPPH and NO scavenging (%). In addition, mineral content was defined with ICP-MS. DPPH scavenging activity (%) was observed most in jujube fruit (80.36%) then it continued as oleaster seed (79.33%) and oleaster fruit (72.00%) and jujube seed (66.26%) respectively. According to protein results, there was a 2.19-fold difference between jujube fruit and seed. This difference was found 0.78 times between oleaster fruit and seed. 4-hydroxybenzoic acid, gallic acid and vanillic acid contents of phenolic compounds were found in high amounts. P (phosphorus) contents in jujube, oleaster, jujube seed and oleaster seed, respectively; 722.67, 736.91, 453.74 and 687.10 mg/kg. In the jujube and jujube seed samples, the contents of Ca (calcium) were found 1261.02 and 2228.24 mg/kg. K (potassium) contents were determined 7351.16 and 7688.42 mg/kg in jujube and oleaster, respectively.

中文翻译:

用色谱法和抗氧化能力测定大枣 (Zizyphus jujuba Mill.) 和油梨 (Elaeagnus angustifolia L.) 甲醇提取物中的植物化学成分含量

摘要 本研究对大枣、越橘果实及其种子的抗氧化作用、蛋白质含量和植物化学结构含量进行了研究。制备了果实和种子的甲醇提取物,挥发性有机成分通过 GC-MS 检测,酚类成分通过 LC-MS/MS 检测。抗氧化活性由 DPPH 和 NO 清除率 (%) 确定。此外,矿物质含量是用 ICP-MS 定义的。DPPH清除活性(%)在枣果中观察到最多(80.36%),然后分别以橄榄果种子(79.33%)和橄榄果(72.00%)和枣种子(66.26%)持续存在。根据蛋白质结果,枣果实和种子之间存在2.19倍的差异。在 oleaster 果实和种子之间发现这种差异是 0.78 倍。4-羟基苯甲酸,酚类化合物的没食子酸和香草酸含量很高。枣、橄榄果、枣种子和橄榄果种子中P(磷)含量分别;722.67、736.91、453.74 和 687.10 毫克/公斤。在枣和枣种子样品中,Ca(钙)含量分别为1261.02和2228.24 mg/kg。枣和橄榄中的 K(钾)含量分别测定为 7351.16 和 7688.42 mg/kg。
更新日期:2020-09-21
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