ABSTRACT

The lack of a unified standard for sampling stage, preservation and measurement of stigma length is the main restriction factor for the study of stigma length trait in rice. In this study, stigma lengths were compared among four different developmental stages (T1-T4) of 30 rice materials under two environments (years) and in 10 measurement periods (D1-D120) of 3 rice materials after treatment with 6 fixatives (S1–S6), and finally the results revealed that the T3 were the best sampling stage and the S6 was the most suitable fixative for stigma length. This study thoroughly describes the process for surveying and measuring stigma length in 6 steps: preparation before sampling, flower sampling, focusing, flower dissection and imaging, obtaining measurements, and data analysis. These findings provide an outline of the methodology for the discovery, fine mapping, and functional analysis of the genes that control the stigma length trait.

摘要

缺乏统一的柱头长度取样阶段、保存和测量标准是水稻柱头长度性状研究的主要制约因素。本研究比较了 30 种水稻材料在两种环境（年）下的 4 个不同发育阶段（T1-T4）和 3 种水稻材料在 6 种固定剂（S1- S6)，最后结果表明，T3 是最好的取样阶段，S6 是最适合柱头长度的固定剂。本研究通过6个步骤详细描述了柱头长度的测量和测量过程：采样前的准备、花卉采样、聚焦、花卉解剖和成像、获取测量值和数据分析。这些发现概述了发现的方法论，