Abstract

The strains 17E-042, 17E-039, and NC13-171 belong to Ascomycota and were isolated from soil collected from Sancheong-gun and Yeongam-gun, Korea. The strain 17E-042 produced white mycelial colonies that developed a sienna color with a round margin on potato dextrose agar (PDA), and the reverse side developed a light sienna color. Morphologically, this strain was similar to the strains of Arthrinium phragmites and A. hydei, but the shorter conidial size of the newly identified strain (17E-042) was distinct. The strain 17E-039 produced macroconidia that were pale yellow to orange-brown, elongated-ellipsoid to oblong, round at both ends, primarily straight but sometimes slightly curved, 0-septate, thin-walled, and filled with numerous droplets, having diameters of 20.4–34.3 × 8.0–12.0 μm. And the strain NC13-171 formed hyaline to light brown chlamydospores, solitary or in a chain. Multigene phylogenetic analyses were conducted using sequence data obtained from internal transcribed spacer (ITS) regions, 28S rDNA large subunit (LSU), β-tubulin (TUB2), translation elongation factor 1-alpha (TEF1-α), and RNA polymerase II large subunit (RPB2) genes. The results of molecular phylogeny, the detailed descriptions and illustrations of each species strongly support our proposal that these strains from soil in Korea be designated as Arthrinium minutisporum sp. nov. and two new records of Pezicula neosporulosa and Acrocalymma pterocarpi.

摘要

菌株17E-042、17E-039和NC13-171属于子囊菌，并从韩国山清郡和灵岩郡的土壤中分离得到。菌株17E-042产生白色菌丝体菌落，在马铃薯葡萄糖琼脂（PDA）上呈a色，边缘呈圆形，而反面则呈淡enna色。从形态上讲，该菌株类似于芦苇和芦苇的菌株。，但是新鉴定出的菌株（17E-042）的分生孢子尺寸较短，这是很明显的。菌株17E-039产生的大分生孢子体呈淡黄色至橙棕色，细长的椭圆体至长圆形，两端均为圆形，主要是笔直但有时稍微弯曲，0分隔，薄壁，并充满了许多直径的液滴20.4–34.3×8.0–12.0μm。NC13-171菌株从透明到淡棕色的衣原体孢子形成单根或链状的透明质酸。使用从内部转录间隔区（ITS），28S rDNA大亚基（LSU），β-微管蛋白（TUB2），翻译延伸因子1-alpha（TEF1-α）和RNA聚合酶II大序列获得的序列数据进行多基因系统发育分析亚基（RPB2）基因。分子系统发育学的结果，每个物种的详细描述和插图都强烈支持我们的建议，即将韩国土壤中的这些菌株命名为Arthrinium minutisporum sp。十一月 和两个新记录的Pezicula neosporulosa和Acrocalymma pterocarpi。