Abstract

Skin damage can be induced by excessive ultraviolet B (UV-B) irradiation. This study aimed to investigate the potential protective activity of persimmon oligo-proanthocyanidins (P-OPC) against UV-B induced human keratinocyte cells (HaCaT cells) and skin damage and its underlying mechanisms in vitro and in vivo. P-OPC was shown to inhibit the production of intracellular reactive oxygen species (ROS) induced by UVB radiation in both HaCaT cells and mouse skin tissues by increasing the activity of the antioxidant enzyme system [superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and glutathione (GSH)]. Furthermore, P-OPC was found to suppress cell apoptosis and the production of inflammatory cytokines, TNF-α, and IL-6. Overall, P-OPC could protect skin tissues from UV-B-induced damage by suppressing oxidant stress, acute inflammation, and cell apoptosis via regulating MAPK and NF-κB signalling pathways. These results indicate the potential of P-OPC as a photochemo-protective agent against UV-B induced skin damage.

摘要

过度的紫外线 B (UV-B) 照射会导致皮肤损伤。本研究旨在研究柿子低聚原花青素 (P-OPC) 对 UV-B 诱导的人角质形成细胞 (HaCaT 细胞) 和皮肤损伤的潜在保护活性及其体外和体内潜在机制. P-OPC 通过增加抗氧化酶系统 [超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH- Px) 和谷胱甘肽 (GSH)]。此外，发现 P-OPC 可抑制细胞凋亡和炎性细胞因子、TNF-α 和 IL-6 的产生。总体而言，P-OPC 可以通过调节 MAPK 和 NF-κB 信号通路抑制氧化应激、急性炎症和细胞凋亡，从而保护皮肤组织免受 UV-B 诱导的损伤。这些结果表明 P-OPC 作为光化学保护剂对抗 UV-B 诱导的皮肤损伤的潜力。