Abstract

Type 2 diabetes is the most prevalent metabolic disease worldwide. The disease is characterised by high blood glucose levels and recently it has been shown that changes in the plasma levels of several miRNAs (miRNA) are associated with the disease. Interestingly, alterations in circulating miRNAs occur years before the onset of the disease and demonstrate predictive power. In this study, we carried out RT-qPCR to examine the plasma levels of two type 2 diabetes specific miRNAs, miR-30d-5p and miR-126-3p in an Iranian population of non-diabetic control individuals, subjects with intermediate hyperglycaemia and type 2 diabetic individuals with hyperglycaemia. We found that the plasma levels of miR-30d and miR-126 increase by 3.1 and 11.16 times, respectively, in individuals with intermediate hyperglycaemia compared to non-diabetic controls. However, no significant changes in the expression of these two miRNAs have been observed between type 2 diabetic individuals and non-diabetic subjects. Our results confirm that alterations in the plasma levels of miR-30d-5p and miR-126-3p could be used as diagnostic markers of type 2 diabetes in the Iranian population as well.

摘要

2 型糖尿病是全世界最普遍的代谢性疾病。该疾病的特点是高血糖水平，最近表明，几种 miRNA (miRNA) 血浆水平的变化与该疾病有关。有趣的是，循环 miRNA 的改变发生在疾病发作前数年，并显示出预测能力。在这项研究中，我们进行了 RT-qPCR 来检查伊朗非糖尿病对照人群、中度高血糖症受试者和患有高血糖症的 2 型糖尿病患者。我们发现，与非糖尿病对照组相比，中度高血糖患者的 miR-30d 和 miR-126 血浆水平分别增加了 3.1 倍和 11.16 倍。然而，在 2 型糖尿病个体和非糖尿病受试者之间未观察到这两种 miRNA 表达的显着变化。我们的结果证实，miR-30d-5p 和 miR-126-3p 血浆水平的改变也可用作伊朗人群中 2 型糖尿病的诊断标志物。