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New insights into AtNBR1 as a selective autophagy cargo receptor in Arabidopsis
Plant Signaling & Behavior ( IF 2.8 ) Pub Date : 2020-10-30 , DOI: 10.1080/15592324.2020.1839226
Youshun Lin 1 , Rongfang Guo 1, 2 , Changyang Ji 1 , Jun Zhou 1, 3 , Liwen Jiang 1, 4
Affiliation  

ABSTRACT Selective autophagy, mediated by cargo receptors and recruiting specific targets to autophagosomes for degradation and recycling, plays an important role in quality control and cellular homeostasis in eukaryotes. The Arabidopsis AtNBR1 shares a similar domain organization with the mammalian autophagic receptors p62 and NBR1. We recently demonstrated that AtNBR1 functions as a selective autophagy receptor for the exocyst component AtExo70E2, a marker for the Exocyst-positive organelle (EXPO), which was achieved via a specific ATG8-AtNBR1-AtExo70E2 interaction in Arabidopsis. Here we further showed that nbr1 CRISPR mutants exhibit an early senescence phenotype under short-day growth conditions, which can be restored by complementation with expression of AtNBR1pro::AtNBR1-GFP in the mutant. Interestingly, in addition to the typical cytosolic and punctate patterns, YFP-AtNBR1 also exhibited a microtubule pattern particularly in the cortical layer. Treatments with the microtubule depolymerizer oryzalin but not the microfilament depolymerizer latrunculin B abolished the microtubule pattern and affected the vacuolar delivery of YFP-AtNBR1 upon autophagy induction. These results indicated that microtubules may be required for AtNBR1 to shuttle its cargos to the vacuole during plant autophagy. The present study thus sheds new light on the recognition and movement pattern of AtNBR1 in selective autophagy in Arabidopsis.

中文翻译:

对 AtNBR1 作为拟南芥中选择性自噬货物受体的新见解

摘要 选择性自噬,由货物受体介导并将特定目标招募到自噬体进行降解和回收,在真核生物的质量控制和细胞稳态中起着重要作用。拟南芥 AtNBR1 与哺乳动物自噬受体 p62 和 NBR1 共享一个类似的域组织。我们最近证明了 AtNBR1 作为 Exocyst 成分 AtExo70E2 的选择性自噬受体发挥作用,AtExo70E2 是 Exocyst 阳性细胞器 (EXPO) 的标记,这是通过拟南芥中的特定 ATG8-AtNBR1-AtExo70E2 相互作用实现的。在这里,我们进一步表明 nbr1 CRISPR 突变体在短日照生长条件下表现出早期衰老表型,这可以通过与突变体中 AtNBR1pro::AtNBR1-GFP 的表达互补来恢复。有趣的是,除了典型的细胞质和点状模式外,YFP-AtNBR1 还表现出微管模式,尤其是在皮质层。用微管解聚剂 oryzalin 而不是微丝解聚剂 latrunculin B 处理消除了微管模式并影响了自噬诱导后 YFP-AtNBR1 的液泡传递。这些结果表明,在植物自噬过程中,AtNBR1 可能需要微管将其货物运送到液泡。因此,本研究为拟南芥选择性自噬中 AtNBR1 的识别和运动模式提供了新的思路。用微管解聚剂 oryzalin 而不是微丝解聚剂 latrunculin B 处理消除了微管模式并影响了自噬诱导后 YFP-AtNBR1 的液泡传递。这些结果表明,在植物自噬过程中,AtNBR1 可能需要微管将其货物运送到液泡。因此,本研究为拟南芥选择性自噬中 AtNBR1 的识别和运动模式提供了新的思路。用微管解聚剂 oryzalin 而不是微丝解聚剂 latrunculin B 处理消除了微管模式并影响了自噬诱导后 YFP-AtNBR1 的液泡传递。这些结果表明,在植物自噬过程中,AtNBR1 可能需要微管将其货物运送到液泡。因此,本研究为拟南芥选择性自噬中 AtNBR1 的识别和运动模式提供了新的思路。
更新日期:2020-10-30
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