Abstract

To investigate the effects of formononetin on rats with gastric ulcer and further to explore its possible mechanism. Rats were randomly divided into sham operation group (Sham), model group (Model), omeprazole control group (Omeprazole) and formononetin in different dose groups (FOR-L, FOR-M, FOR-H). Rats model with gastric ulcer were established by 100% glacial acetic acid. Hematoxylin–eosin (H&E) staining was used to observe the pathological morphology of gastric mucosa. Immunohistochemistry and enzyme-linked immunosorbent assay (ELISA) were used to detect the level of inflammatory and angiogenesis related factors. The expressions of nuclear factor kappa-B (NF-κB) signaling pathway-related proteins were detected by western blot. Formononetin and omeprazole could ameliorate the pathological morphology of gastric mucosa in gastric ulcer rats. Compared with Model group, the levels of tumor necrosis factor (TNF)-α, Interleukin (IL)-1β, IL-6, myeloperoxidase (MPO), human endothelin (ET)-1 and p-P65 protein in formononetin treatment and omeprazole groups were significantly decreased (p < 0.05). Moreover, formononetin could increase the content of vascular endothelial growth factor (VEGF), nitric oxide (NO) and the levels of CD34, tight junction proteins (ZO-1 and occludin) and p-IκBα in a dose-dependent manner. Formononetin can ameliorate gastric ulcer in rats by inhibiting inflammation and promoting gastric mucosal angiogenesis, and its mechanism maybe related to NF-κB signaling pathway.

摘要

探讨芒柄花素对胃溃疡大鼠的作用，并进一步探讨其可能的作用机制。将大鼠随机分为假手术组（Sham）、模型组（Model）、奥美拉唑对照组（Omeprazole）和不同剂量组（FOR-L、FOR-M、FOR-H）的芒柄花素。100%冰醋酸建立大鼠胃溃疡模型。苏木精-伊红（H&E）染色用于观察胃黏膜的病理形态。免疫组化和酶联免疫吸附试验（ELISA）用于检测炎症和血管生成相关因子的水平。Western blot检测核因子κ-B（NF-κB）信号通路相关蛋白的表达。芒柄花素和奥美拉唑可改善胃溃疡大鼠胃黏膜的病理形态。p  < 0.05)。此外，芒柄花素能以剂量依赖性方式增加血管内皮生长因子（VEGF）、一氧化氮（NO）的含量以及CD34、紧密连接蛋白（ZO-1和occludin）和p-IκBα的水平。芒柄花素通过抑制炎症和促进胃黏膜血管生成改善大鼠胃溃疡，其作用机制可能与NF-κB信号通路有关。