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Optimization of antioxidant activity and bioactive compounds extraction of winter savory leaves by high hydrostatic pressure
High Pressure Research ( IF 1.2 ) Pub Date : 2020-10-01 , DOI: 10.1080/08957959.2020.1830079
Sílvia A. Moreira 1, 2 , Manuela E. Pintado 2 , Jorge A. Saraiva 1
Affiliation  

ABSTRACT Winter savory is often used in traditional medicine, having several recognized biological properties. This study aimed to evaluate the effect of high pressure-assisted extraction (HPE) and its optimization by response surface methodology to obtain winter savory extracts with a high content of bioactive compounds and high antioxidant activity (the effect of extraction pressure, extraction time, and ethanol concentration were investigated). Results showed that data could be well fitted to second-order polynomial mathematical models for total phenolics, total flavonoids, chlorophylls, carotenoids, and extraction yield, as well as for antioxidant activity. The models predicted optimal conditions ranging 200–500 MPa, extraction time 1–20 min, and ethanol concentration 0–70% (v,v). In comparison with extraction at atmospheric pressure, HPE showed to be more efficient, allowing obtaining an increase of about 40% for all compounds, and an increase of 29, 48, and 70% for antioxidant activity by FRAP, DPPH and ABTS assays, respectively. GRAPHICAL ABSTRACT

中文翻译:

高静水压优化冬香叶抗氧化活性及生物活性成分提取工艺

摘要 冬香常用于传统医学,具有多种公认的生物学特性。本研究旨在评估高压辅助提取 (HPE) 的效果及其通过响应面方法优化以获得具有高生物活性化合物含量和高抗氧化活性的冬香提取物(提取压力、提取时间和乙醇浓度进行了调查)。结果表明,数据可以很好地拟合总酚类、总黄酮类、叶绿素、类胡萝卜素和提取率以及抗氧化活性的二阶多项式数学模型。该模型预测的最佳条件范围为 200-500 MPa,提取时间为 1-20 分钟,乙醇浓度为 0-70% (v,v)。与常压萃取相比,HPE 显示出更有效,通过 FRAP、DPPH 和 ABTS 测定,所有化合物均能增加约 40%,抗氧化活性分别增加 29、48 和 70%。图形概要
更新日期:2020-10-01
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