ABSTRACT

A high glucose (HG) or state predispose to osteoarthritis and has adverse effects on cartilage regeneration. Human Wharton’s jelly stem cells (hWJSCs) isolated from umbilical cords have been reported to promote cartilage regeneration. Furthermore, resveratrol (RSV) has numerous beneficial effects including anti-inflammatory properties. Therefore, we investigated the effects of HG (28 mM), RSV (17 µM), and both (HG-RSV) on chondrogenesis of hWJSCs as assessed by proteoglycan deposition, proliferation, and pro-inflammatory cytokine production compared with the control (5 mM glucose). Toluidine blue staining of proteoglycans was positive under all conditions. HG enhanced the growth of differentiated cells (DCs) on days 14 and 21. Growth of DCs were decreased on day 4, but increased on days 14 and 21, following treatment with RSV and HG-RSV. Compared with the HG group, HG-RSV decreased the proliferation of DCs on days 4 and 14. COLI, COLII, and COLX transcripts were increased, while SOX9 transcript was decreased in all treated groups. IL-1α, IL-6 and IL-8 levels were decreased in RSV and HG-RSV groups. The levels of G-CSF and GM-CSF were high in HG-RSV group, while HG reduced the GM-CSF level. Thus, under HG conditions, RSV promotes chondrogenesis by increasing proteoglycans and collagen synthesis as well as by regulating the pro-inflammatory cytokines.

摘要

高血糖（HG）或状态易诱发骨关节炎，并且对软骨再生产生不利影响。据报道，从脐带分离出的人类沃顿氏胶冻干细胞（hWJSC）可以促进软骨再生。此外，白藜芦醇（RSV）具有许多有益的作用，包括抗炎特性。因此，我们通过蛋白聚糖的沉积，增殖和促炎性细胞因子的产生来评估与对照相比，HG（28 mM），RSV（17 µM）和两者（HG-RSV）对hWJSC软骨形成的影响（5）。 mM葡萄糖）。蛋白聚糖的甲苯胺蓝染色在所有条件下均为阳性。在用RSV和HG-RSV处理后，HG在第14天和第21天促进了分化细胞（DC）的生长。在第4天，DC的生长减少了，但在第14天和21天增加了。与HG组相比，HG-RSV在第4天和第14天降低了DC的增殖。在所有处理组中，COLI，COLII和COLX转录本均增加，而SOX9转录本则下降。RSV和HG-RSV组的IL-1α，IL-6和IL-8水平降低。HG-RSV组的G-CSF和GM-CSF含量较高，而HG降低了GM-CSF含量。因此，在HG条件下，RSV通过增加蛋白聚糖和胶原蛋白的合成以及调节促炎性细胞因子来促进软骨形成。而HG降低了GM-CSF的水平。因此，在HG条件下，RSV通过增加蛋白聚糖和胶原蛋白的合成以及调节促炎性细胞因子来促进软骨形成。而HG降低了GM-CSF的水平。因此，在HG条件下，RSV通过增加蛋白聚糖和胶原蛋白的合成以及调节促炎性细胞因子来促进软骨形成。