ABSTRACT

Oxidative stress from the trophoblasts is one of the possible pathological mechanisms of Preeclampsia (PE). This study aimed at exploring the potential effects of astaxanthin (ATX) on oxidative stress damaged placental trophoblast cell line HTR-8/SVneo. Oxidative stress-induced damaged through H₂O₂ treatment was checked by MTS CellTiter 96® cell viability, 2ʹ,7ʹ-dichlorofluorescein diacetate (DCFH-DA) induced fluorescence, the level of the intracellular malondialdehyde (MDA), and the detection of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT). Different concentrations of ATX were applied, and then the proliferation rate, apoptotic percentage, cell cycle distribution, invasion test and relative biological function of the rescued cells were followed. We provide evidence that ATX had an anti-oxidative effect against oxidative stress induced by H₂O₂ on the trophoblast cell line and had beneficial role in promoting cell proliferation, inhibiting cell apoptosis, and inducing cell invasion.

Abbreviations: UV: ultraviolet; DCFH-DA: 2’,7’-dichlorofluorescein diacetate; EVT: extravillous trophoblast; MMPs: matrix metalloproteinases; IUGR: intrauterine growth restriction.

摘要

来自滋养层细胞的氧化应激是先兆子痫 (PE) 的可能病理机制之一。本研究旨在探索虾青素 (ATX) 对氧化应激损伤的胎盘滋养层细胞系 HTR-8/SVneo 的潜在影响。H ₂ O ₂氧化应激引起的损伤通过 MTS CellTiter 96® 细胞活力、2ʹ,7ʹ-二氯荧光素二乙酸酯 (DCFH-DA) 诱导的荧光、细胞内丙二醛 (MDA) 的水平以及谷胱甘肽过氧化物酶 (GSH-Px)、超氧化物歧化酶 ( SOD) 和过氧化氢酶 (CAT)。应用不同浓度的ATX，然后观察获救细胞的增殖率、凋亡百分比、细胞周期分布、侵袭试验和相关生物学功能。我们提供的证据表明，ATX 对 H ₂ O ₂诱导的滋养层细胞系的氧化应激具有抗氧化作用，并在促进细胞增殖、抑制细胞凋亡和诱导细胞侵袭方面具有有益作用。

缩写：UV：紫外线；DCFH-DA：2',7'-二氯荧光素二乙酸酯；EVT：绒毛外滋养层；MMPs：基质金属蛋白酶；IUGR：宫内生长受限。