Abstract In this study, DNA-functionalize-magnetic beads were investigated as sorbent materials for effective removing 1,2-benzanthracene (BaA) from water. In order to reveal the removal mechanism, the interaction mode between BaA and DNA was evaluated by using various characterization tools such as UV-visible and circular dichroism spectroscopy, fluorescence and resonance scattering spectroscopy, and agarose gel electrophoresis. In the presence of BaA, the melting temperature of DNA increased from 76.2 °C to 82.3 °C, which closely related to the intercalating of BaA. It was found that a part of the ethidium bromide (EB) binding sites to DNA were occupied by BaA in EB competing study. The results indicated that a new complex appeared between hsDNA and BaA, and the number of the binding sites (n) and the binding constants (KA) at different temperatures were obtained. DNA binding saturation value (≈0.80) was obtained by resonance scattering spectra study. BaA could be enriched and removed by DNA-functionalize-magnetic beads via the intercalation, and the removal efficiency was 97.73% when the initial concentration was 2.45 x10−6 mol·L−1 (559.31 μg/L).

中文翻译：

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通过插入 1,2-苯并蒽与 DNA 和基于磁珠的分离去除 1,2-苯并蒽

摘要 在本研究中，研究了 DNA 功能化磁珠作为吸附材料，可有效去除水中的 1,2-苯并蒽 (BaA)。为了揭示去除机制，通过使用各种表征工具，如紫外-可见光和圆二色光谱、荧光和共振散射光谱以及琼脂糖凝胶电泳，评估了 BaA 和 DNA 之间的相互作用模式。在 BaA 存在下，DNA 的解链温度从 76.2 °C 升高到 82.3 °C，这与 BaA 的嵌入密切相关。在EB竞争研究中发现，部分溴化乙锭（EB）与DNA的结合位点被BaA占据。结果表明 hsDNA 和 BaA 之间出现了一个新的复合物，得到不同温度下的结合位点数（n）和结合常数（KA）。DNA结合饱和值（≈0.80）是通过共振散射光谱研究获得的。DNA-功能化-磁珠可通过嵌入富集和去除BaA，初始浓度为2.45 x10-6 mol·L-1 (559.31 μg/L)时去除效率为97.73%。