ABSTRACT

Troxerutin is a natural flavonoid present abundantly in tea, coffee, olives, wheat, and a variety of fruits and vegetables. Due to its diverse pharmacological properties, this flavonoid has aroused interest for treatment of various diseases, and consequently prompted investigation into its toxicological characteristics. The aim of this study was to evaluate the genotoxic and mutagenic effects and chemoprotective activity attributed to troxerutin using human peripheral blood leukocytes (PBLs) through several well-established experimental protocols based upon different parameters. Data demonstrated that troxerutin (100 to 1000 µM) induced no marked cytotoxic effect on PBLs after 24 hr, and did not produce strand breaks and mutagenicity. Regarding chemoprevention, this flavonoid attenuated cytotoxicity, genotoxicity, and mutagenicity initiated by hydrogen peroxide (H₂O₂) in human PBLs. Further, troxerutin demonstrated no marked cytotoxic effect on PBLs and exerted a protective effect against oxidative stress induced by H₂O₂ through modulation of GSH-dependent enzymes.

摘要

Troxerutin 是一种天然黄酮类化合物，大量存在于茶、咖啡、橄榄、小麦和各种水果和蔬菜中。由于其多种药理特性，这种黄酮类化合物已引起人们对治疗各种疾病的兴趣，并因此促使对其毒理学特征的研究。本研究的目的是通过基于不同参数的几种完善的实验方案，使用人外周血白细胞 (PBL) 评估归因于曲克芦丁的基因毒性和致突变作用以及化学保护活性。数据表明曲克芦丁（100 至 1000 µM）在 24 小时后对 PBL 没有产生明显的细胞毒性作用，并且不会产生链断裂和致突变性。关于化学预防，这种类黄酮减弱了细胞毒性、基因毒性、₂ O ₂ ) 在人类 PBL 中。此外，曲克芦丁对 PBL 没有显着的细胞毒性作用，并通过调节 GSH 依赖性酶对 H ₂ O ₂诱导的氧化应激发挥保护作用。