Abstract

Aim

The preparation of thiolated okra gum by use of full factorial design to optimise the reaction conditions.

Methods

Thiolated gum was obtained by esterification optimised by full factorial design. The effect of varying the thiolated Okra concentration, chitosan concentration, pH, and stirring speed on particle size, entrapment efficiency and zeta potential was observed using Box–Behnken design.

Results

Maximum yield and degree of substitution were obtained at reaction time of 152 min, 6.73 ml of thioglycolic acid and 70 °C of temperature. The optimised calculated parameters were thiolated okra concentrations of (0.07% w/v), chitosan concentration (0.05% w/v), pH (3), stirring speed (4430 rpm), which yielded nanoparticles of size 294.3 ± 0.3 nm, 43.57 ± 1.21% entrapment and 23.29 ± 2.3 mV of Zeta potential.

Conclusion

NPs were observed to be promising for brain targeting.

中文翻译：

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硫醇化秋葵壳聚糖纳米粒：作为鼻内给药剂的制备和优化

摘要

目的

采用全析因设计优化反应条件制备硫醇化秋葵胶。

方法

硫醇化胶是通过全因子设计优化的酯化获得的。使用 Box-Behnken 设计观察了硫醇化秋葵浓度、壳聚糖浓度、pH 值和搅拌速度对粒径、包封率和 zeta 电位的影响。

结果

在 152 分钟的反应时间、6.73 ml 巯基乙酸和 70 °C 的温度下获得最大产率和取代度。优化的计算参数是硫醇化秋葵浓度 (0.07% w/v)、壳聚糖浓度 (0.05% w/v)、pH (3)、搅拌速度 (4430 rpm)，产生尺寸为 294.3 ± 0.3 nm、43.57 的纳米颗粒± 1.21% 截留率和 23.29 ± 2.3 mV Zeta 电位。

结论

观察到 NPs 有望用于大脑靶向。