ABSTRACT The core objective of the current study was to assess the effect of encapsulation on the viability and stability of probiotic bacteria under simulated gastrointestinal digestion and thermal conditions. Purposely, probiotics were encapsulated with hydrogel matrices (sodium alginate and carrageenan) using encapsulator. Furthermore, developed microbeads were characterized by X-ray diffraction (XRD) and Fourier Transform Infrared (FTIR) spectroscopy to elucidate the interaction between hydrogel matrices and probiotics. The viability of probiotics assessed by subjecting to simulated GIT and thermal conditions. Encapsulation exhibited a significant (p< .05) effect on the survival and stability of probiotics. Overall, a decreasing trend in viability of probiotics was observed in all treatments. A rapid log reduction was observed when free probiotic cells were stored at refrigeration temperature compared to encapsulated probiotic bacteria. Likewise, in vitro gastrointestinal assay, only 3 log while in case of non-encapsulated bacteria 6 log reduction was recorded. In short, the results of the viable count in the case of encapsulated cells were above recommended level (106 cfu/g) under thermal as well as in GIT conditions.

中文翻译：

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模拟胃肠道和热条件下游离和封装益生菌的存活和稳定性

摘要 本研究的核心目标是评估在模拟胃肠消化和热条件下封装对益生菌活力和稳定性的影响。特意使用封装器将益生菌与水凝胶基质（海藻酸钠和角叉菜胶）封装在一起。此外，开发的微珠通过 X 射线衍射 (XRD) 和傅立叶变换红外 (FTIR) 光谱进行表征，以阐明水凝胶基质和益生菌之间的相互作用。通过模拟 GIT 和热条件评估益生菌的生存能力。封装对益生菌的存活和稳定性有显着的影响 (p < .05)。总体而言，在所有处理中都观察到益生菌的生存力呈下降趋势。与封装的益生菌相比，当游离益生菌细胞在冷藏温度下储存时，观察到对数迅速减少。同样，在体外胃肠道测定中，仅记录了 3 log，而在非包囊细菌的情况下，记录了 6 log 减少。简而言之，在热和 GIT 条件下，封装细胞的活菌计数结果高于推荐水平 (106 cfu/g)。