ABSTRACT

We investigated lateral thoracic and posterior thigh perforator flaps for viability, vascularization, perfusion and apoptosis in a rat model. Wistar albino rats were divided into six groups: lateral thoracic artery perforator flap (LTPF) sham, 3 × 2 cm² LTPF, 3 × 6 cm² LTPF, posterior thigh perforator flap (PTPF) sham, 3 × 2 cm² PTPF, and 3 × 6 cm² PTPF. Flap viability was determined on postoperative days 1 and 7. On day 7, flaps were photographed and their viability was measured using two-dimensional planimeter paper. Tissue samples were harvested for examination by histology and immunohistochemistry. Viability differences were statistically significant. Epithelial thickness, vascularity and number of fibroblasts were reduced in the 3 × 6 cm² groups. Neovascularization and apoptosis based on molecular tests were not significantly different among groups. Flap size and location are important factors for closure of surgical or traumatic defects. We suggest that for clinical application, wound complications will occur less frequently with perforators that nourish large areas of flaps.

摘要

我们在大鼠模型中研究了胸外侧和大腿后部穿支皮瓣的活力、血管化、灌注和细胞凋亡。Wistar 白化大鼠分为六组：胸外侧动脉穿支皮瓣（LTPF）假手术，3 × 2 cm ² LTPF，3 × 6 cm ² LTPF，大腿后部穿支皮瓣（PTPF）假手术，3 × 2 cm ² PTPF，和3 × 6 厘米²PTPF。在术后第 1 天和第 7 天测定皮瓣活力。在第 7 天，对皮瓣拍照并使用二维平面纸测量其活力。收集组织样品用于组织学和免疫组织化学检查。生存力差异具有统计学意义。3 × 6 cm ²组的上皮厚度、血管分布和成纤维细胞数量减少。基于分子测试的新生血管形成和细胞凋亡在各组之间没有显着差异。皮瓣大小和位置是闭合手术或外伤缺损的重要因素。我们建议在临床应用中，穿孔器滋养大面积皮瓣时，伤口并发症的发生率会降低。