Traditional large‐scale culture systems for human mesenchymal stem/stromal cells (hMSCs) use solid microcarriers as attachment substrates. Although the use of such substrates is advantageous because of the high surface‐to‐volume ratio, cell harvest from the same substrates is a challenge as it requires enzymatic treatment, often combined with agitation. Here, we investigated a two‐phase system for expansion and non‐enzymatic recovery of hMSCs. Perfluorocarbon droplets were dispersed in a protein‐rich growth medium and were used as temporary liquid microcarriers for hMSC culture.

中文翻译：

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在临时液体微载体上扩增人间充质干/基质细胞

用于人类间充质干/基质细胞（hMSC）的传统大规模培养系统使用固体微载体作为附着底物。尽管由于高的表面体积比，使用这种底物是有利的，但是从相同的底物收获细胞是一个挑战，因为它需要酶处理，通常与搅拌相结合。在这里，我们研究了用于hMSC扩增和非酶促回收的两阶段系统。全氟化碳液滴分散在富含蛋白质的生长培养基中，并用作hMSC培养的临时液体微载体。