This study systematically investigated the effects of phytoestrogen glabrene on postmenopausal osteoporosis in an ovariectomy (OVX) rat model. Glabrene administration (25, 50, and 100 mg/kg) for 13 weeks can significantly slow down the body weight gain and slightly increase the uterus weight of OVX rats. The increased levels of U‐Ca, U‐P levels, urine DPD/creatinine, serum ALP, OCN, triglycerides, and total cholesterol induced by OVX were dramatically inhibited in rats, whereas no difference occurred for S‐Ca and S‐P in all groups. Furthermore, glabrene can enhance bone mineral density of the right femur, fourth‐lumbar vertebra and tibia and improve biomechanical parameters, such as femoral neck loading force, three‐point bending of the tibia, and vertebral compression in OVX rats. Moreover, glabrene greatly suppressed the expression of TRAP protein but increased OPG and BGP protein expression in tibia tissue of OVX rats. In addition, OVX‐induced reduction of Lrp‐5, β‐catenin, Runx2, and Osx protein expression was all restored by glabrene treatment. The present study indicated that glabrene might be a potential alternative medicine for the prevention and treatment of postmenopausal osteoporosis via activation of the Wnt/β‐catenin signaling pathway.

中文翻译：

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植物雌激素基布伦通过上调Wnt /β-catenin信号传导通路来预防卵巢切除大鼠的骨质疏松

这项研究系统地研究了植物雌激素对小鼠卵巢切除（OVX）模型中绝经后骨质疏松的影响。给予谷蛋白（25、50和100 mg / kg）13周可显着减慢OVX大鼠的体重增加并略微增加子宫重量。OVX诱导的大鼠U-Ca，U-P水平，尿DPD /肌酐，血清ALP，OCN，甘油三酸酯和总胆固醇的升高被显着抑制，而S-Ca和S-P在大鼠中没有差异。所有组。此外，格柏能增强OVX大鼠的右股骨，第四腰椎和胫骨的骨矿物质密度，并改善生物力学参数，例如股骨颈负重力，胫骨的三点弯曲和椎骨压缩。此外，千里光能显着抑制OVX大鼠胫骨组织TRAP蛋白的表达，但增加OPG和BGP蛋白的表达。此外，OVX诱导的Lrp-5，β-catenin，Runx2和Osx蛋白表达的降低均通过上蜡处理得以恢复。本研究表明，通过激活Wnt /β-catenin信号通路，格柏烯可能是预防和治疗绝经后骨质疏松症的潜在替代药物。