Functionalized peptide hydrogels as tunable extracellular matrix mimics for biological applications,Peptide Science

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Functionalized peptide hydrogels as tunable extracellular matrix mimics for biological applications
Peptide Science ( IF 1.5 ) Pub Date : 2020-10-26 , DOI: 10.1002/pep2.24201
Katharina S. Hellmund ₁ , Benjamin Lospichl ₂ , Christoph Böttcher ₃ , Kai Ludwig ₃ , Uwe Keiderling ₄ , Laurence Noirez ₅ , Annika Weiß ₁ , Dorian J. Mikolajczak ₁ , Michael Gradzielski ₂ , Beate Koksch ₁

Affiliation

The development of tailorable and biocompatible three‐dimensional (3D) substrates or molecular networks that reliably mimic the extracellular matrix (ECM) and influence cell behavior and growth in vitro is of increasing interest for cell‐based applications in the field of tissue engineering and regenerative medicine. In this context, we present a novel coiled coil‐based peptide that self‐assembles into a 3D‐α‐helical fibril network and functions as a self‐supporting hydrogel. By functionalizing distinct coiled‐coil peptides with cellular binding motifs or carbohydrate ligands (mannose), and by utilizing the multivalency and modularity of coiled‐coil assemblies, tailored artificial ECMs are obtained. Fibrillar network and ligand density, as well as ligand composition can readily be adjusted by changes in water content or peptide concentrations, respectively. Mesoscopic structure of these networks was assessed by rheology and small‐angle neutron scattering experiments. Initial cell viability studies using NIH/3T3 cells showed comparable or even superior cell viability using the presented artificial ECMs, compared to commercially available 3D‐cell culture scaffold Matrigel. The herein reported approach presents a reliable (low batch‐to‐batch variation) and modular pathway toward biocompatible and tailored artificial ECMs.

中文翻译：

功能化肽水凝胶可作为生物学应用中的可调节细胞外基质模拟物

可定制且生物相容的3D（3D）底物或分子网络的开发，这些底物或分子网络可靠地模拟了细胞外基质（ECM）并在体外影响细胞行为和生长在组织工程和再生医学领域中，基于细胞的应用越来越引起人们的关注。在此背景下，我们提出了一种新型的基于卷曲螺旋的肽，该肽可自组装成3D-α-螺旋原纤维网络，并起自支撑水凝胶的作用。通过用细胞结合基序或碳水化合物配体（甘露糖）功能化不同的卷曲螺旋肽，并利用卷曲螺旋装配的多价性和模块化，获得了量身定制的人工ECM。纤丝网络和配体密度以及配体组成可以分别通过水含量或肽浓度的变化容易地调节。通过流变学和小角度中子散射实验评估了这些网络的介观结构。与市场上可买到的3D细胞培养支架基质胶相比，使用NIH / 3T3细胞进行的初始细胞活力研究表明，使用本文提出的人工ECM可以比较甚至更高的细胞活力。本文报道的方法为生物相容性和量身定制的人工ECM提供了可靠的（批次间差异小）和模块化途径。

更新日期：2020-10-26

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