The aim was to investigate the detection rates of periodontal bacteria (Porphyromonas gingivalis, Tannerella forsythia, Prevotella intermedia, and Aggregatibacter actinomycetemcomitans) and herpesviruses (herpes simplex virus‐1 [HSV‐1], cytomegalovirus [CMV], and Epstein‐Barr virus [EBV]) in different forms and severity of periodontal disease, and to compare them with those in periodontally healthy subjects. One hundred and twenty‐nine patients participated in the study: 39 diagnosed with periodontal abscess (PA), 33 with necrotizing ulcerative periodontitis (NUP), 27 with chronic periodontitis (CP), and 30 participants with healthy periodontal tissue represented a healthy control group. All patients with periodontal disease (PA, NUP, and CP) were also divided into two groups according to the severity of their disease: moderate and severe periodontitis. The subgingival samples were collected from the periodontitis active sites and the detection of microorganisms was performed by end‐point polymerase chain reaction analyses. The results revealed significantly higher detection rates of P. gingivalis, T. forsythia, and P. intermedia in all three groups of patients with periodontitis than in healthy participants. The highest detection rate of A. actinomycetemcomitans was noticed in CP, which was significantly higher than that in PA, NUP, and healthy control. The occurrence of EBV was significantly higher in NUP than in CP and healthy participants. CMV was detected significantly more frequently in PA and NUP than in CP and healthy participants. Comparisons among healthy participants and patients with moderate and severe periodontitis showed significantly higher detection rates of EBV and CMV in patients with severe forms of periodontitis than in healthy participants and those with moderate periodontitis.

中文翻译：

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不同形式牙周疾病中牙周细菌和疱疹病毒的检出率

目的是研究牙周细菌（牙龈卟啉菌，连翘坦氏菌，中间细杆菌和放线杆菌）的检出率。）和疱疹病毒（单纯性疱疹病毒-1 [HSV-1]，巨细胞病毒[CMV]和爱泼斯坦-巴尔病毒[EBV]）以不同的形式和严重程度感染牙周疾病，并将其与牙周健康受试者进行比较。129名患者参加了该研究：39名诊断为牙周脓肿（PA），33名坏死性溃疡性牙周炎（NUP），27名慢性牙周炎（CP）和30名健康牙周组织参与者为健康对照组。所有患牙周疾病的患者（PA，NUP和CP）也根据疾病的严重程度分为两组：中度和重度牙周炎。从牙周炎活动部位收集龈下样品，并通过终点聚合酶链反应分析进行微生物检测。三组牙周炎患者的牙龈卟啉单胞菌，连翘T.连翘和中间媒介均比健康受试者好。CP中发现放线菌的检出率最高，显着高于PA，NUP和健康对照组。NUP中EBV的发生率显着高于CP和健康参与者。在PA和NUP中，检测到CMV的频率明显高于CP和健康参与者。健康受试者与中，重度牙周炎患者的比较表明，重度牙周炎患者中EBV和CMV的检出率明显高于健康受试者和中度牙周炎患者。