Long non‐coding RNA SOX21‐AS1 enhances the stemness of breast cancer cells via the Hippo pathway,FEBS Open Bio

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Long non‐coding RNA SOX21‐AS1 enhances the stemness of breast cancer cells via the Hippo pathway
FEBS Open Bio ( IF 2.8 ) Pub Date : 2020-10-26 , DOI: 10.1002/2211-5463.13015
Lanzhen Li ₁ , Dongmei Meng ₂ , Ruiqing Wang ₃

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Breast cancer stem cells (BCSCs) have high tumorigenicity and invasiveness, which contributes to recurrence and metastasis. The long non‐coding RNA SOX21‐AS1 has been previously reported to modulate the properties of breast cancer stem cells via targeting SOX2, although the underlying molecular mechanisms remain unclear. To investigate this issue, we first confirmed that the expression level of SOX21‐AS1 is increased in breast cancer tissues and cell lines (MCF‐7, MDA‐MB‐231, CSC‐MCF‐7, CSC‐MDA‐MB‐231), especially in BCSCs. We demonstrated that SOX21‐AS1 promotes the stemness of CSC‐MCF‐7 cells through western blot detection of stemness‐related proteins, as well as side population and sphere formation assays. Overexpression of SOX21‐AS1 enhanced the proliferation, migration and invasion of CSC‐MCF‐7 cells. We also observed that SOX21‐AS1 inhibited the Hippo pathway. SOX21‐AS1 enhanced the stemness, migration and invasion of CSC‐MCF‐7 cells by increasing the nuclear localization of YAP and decreasing the level of pYAP. Overall, we conclude that SOX21‐AS1 may promote the stemness viability, proliferation, migration and invasion of BCSCs by inhibiting the Hippo pathway. Our findings provide insights into potential biomarkers and prognostic measures for the treatment of breast cancer.

中文翻译：

长链非编码 RNA SOX21-AS1 通过 Hippo 通路增强乳腺癌细胞的干性

乳腺癌干细胞（BCSCs）具有高致瘤性和侵袭性，有助于复发和转移。此前有报道称，长链非编码 RNA SOX21-AS1 通过靶向 SOX2 来调节乳腺癌干细胞的特性，尽管其潜在的分子机制仍不清楚。为了研究这个问题，我们首先证实了 SOX21-AS1 在乳腺癌组织和细胞系（MCF-7、MDA-MB-231、CSC-MCF-7、CSC-MDA-MB-231）中的表达水平升高，尤其是在 BCSC 中。我们证明 SOX21-AS1 通过干性相关蛋白的蛋白质印迹检测以及侧群和球体形成测定来促进 CSC-MCF-7 细胞的干性。SOX21-AS1的过表达增强了CSC-MCF-7细胞的增殖、迁移和侵袭。我们还观察到 SOX21-AS1 抑制 Hippo 通路。SOX21-AS1 通过增加 YAP 的核定位和降低 pYAP 的水平来增强 CSC-MCF-7 细胞的干性、迁移和侵袭。总体而言，我们得出结论，SOX21-AS1 可能通过抑制 Hippo 通路促进 BCSCs 的干细胞活力、增殖、迁移和侵袭。我们的研究结果为治疗乳腺癌的潜在生物标志物和预后措施提供了见解。

更新日期：2021-01-04

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