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A gene silencing of V‐ATPase subunit A interferes with survival and development of the tomato leafminer, Tuta absoluta
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2020-10-30 , DOI: 10.1002/arch.21753
Shima Rahmani 1 , Ali R. Bandani 2
Affiliation  

RNA interference (RNAi) technology is not only considered as a tool to analyze gene function, but it is also potentially considered as a strategy to develop novel biopesticide. In the current study, a double‐stranded RNA specific to v‐ATPase subunit A of the tomato leafminer, Tuta absoluta (Meyrick; Lepidoptera: Gelechiidae), was orally administered. A gradual decrease in the expression of the gene was observed from Day 1 to 3 and resulted in significant larval mortality. These results suggest that v‐ATPases A can be considered as a promising target gene by RNAi technology to be used in the management of the tomato leafminer.

中文翻译:

V-ATPase A亚基的基因沉默会干扰番茄切叶者Tuta absoluta的生存和发育

RNA干扰(RNAi)技术不仅被视为分析基因功能的工具,而且还潜在地被视为开发新型生物农药的策略。在本研究中,口服给予了番茄切叶者Tuta absoluta(Meyrick;鳞翅目:Gelechiidae)的v-ATPase亚基A特异性的双链RNA 。从第1天到第3天观察到该基因的表达逐渐下降,导致幼虫死亡率显着下降。这些结果表明,v-ATPases A可被RNAi技术视为有前途的靶基因,可用于番茄切叶机的管理。
更新日期:2020-12-26
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