Global Immunometabolic Profiling of AECOPD,Small Methods

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Global Immunometabolic Profiling of AECOPD
Small Methods ( IF 10.7 ) Pub Date : 2020-10-26 , DOI: 10.1002/smtd.202000483
Dongli Song ₁ , Fangming Liu ₁ , Bijun Zhu ₁ , Jun Yin ₂ , Zhongshu Kuang ₂ , Zhimin Dong ₂ , Lei Yan ₂ , Ling Ye ₃ , Yong Zhang ₃ , Zhenju Song ₂ , Duojiao Wu ₁ , Xiangdong Wang ₁

Affiliation

Acute exacerbations of chronic obstructive pulmonary disease (AECOPD) is a major public health issue. In the study, mass cytometry is used with extensive antibody panels to perform in‐depth immune profiling of samples from patients with AECOPD, acute lung infection(AI) and healthy controls. Immune composition of 11 populations including CD4/CD8 T cells, B cells, NK cells, and others is identified. Significantly, 7 T cell subsets are changed among the three groups. Then RNA sequencing at single cell level is further processed to provide a detailed peripheral T cell immunity of AECOPD. Combined analysis suggests that dysfunctional effector T cells in AECOPD are characterized with suppressed mitochondria, dampened mTOR activity, and IFN‐γ production. Furthermore, targeting mTOR pathway synergized the PD‐1 blockade therapy promotes T cell glycolysis and IFN‐γ production. The study presents an in‐depth immunometabolic atlas of AECOPD.

中文翻译：

AECOPD的全球免疫代谢分析

慢性阻塞性肺疾病（AECOPD）的急性加重是主要的公共卫生问题。在这项研究中，大规模流式细胞仪与广泛的抗体组合一起使用，对来自AECOPD，急性肺部感染（AI）和健康对照的患者的样品进行深入的免疫分析。确定了11个种群的免疫组成，包括CD4 / CD8 T细胞，B细胞，NK细胞等。值得注意的是，三组中有7个T细胞亚群发生了变化。然后进一步处理单细胞水平的RNA测序，以提供AECOPD的详细外周T细胞免疫性。组合分析表明，AECOPD中功能性T细胞功能异常的特点是线粒体抑制，mTOR活性减弱和IFN-γ产生。此外，靶向mTOR途径的协同作用PD-1阻断疗法可促进T细胞糖酵解和IFN-γ产生。该研究提供了AECOPD的深入免疫代谢图集。

更新日期：2020-12-03

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