While the identification of microbial eukaryotes using metabarcoding tools is now widespread, additional data are needed to confirm molecular observations, to mark the difference between species and population variants, and to better understand the biogeography of microbial eukaryotes. In this issue of Molecular Ecology, Postel et al not only use three molecular approaches to identify subgroups of Fragilariopsis kerguelensis but also use morphology and physiology to better understand the relationship between the three genotypes. They revealed that (a) the three genotypes of the diatom F. kerguelensis show negligible gene flux; (b) two of the genotypes are geographically isolated with different physiology but still able to crossbreed; and (c) the remaining genotype is omnipresent but reproductively isolated.

中文翻译：

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南大洋硅藻Fragilariopsis kerguelensis内正在进行的物种形成？

虽然现在使用元条形码工具识别微生物真核生物很普遍，但仍需要更多数据来确认分子观测结果，标记物种和种群变异之间的差异以及更好地了解微生物真核生物的生物地理。在本期《分子生态学》中，Postel等人不仅使用三种分子方法来鉴定克雷格氏菌的亚组，而且还使用形态学和生理学来更好地了解这三种基因型之间的关系。他们发现（a）硅藻F.kerguelensis的三种基因型显示微不足道的基因通量；（b）两种基因型在地理上是分离的，具有不同的生理学，但仍然能够杂交；（c）其余基因型无所不在，但生殖分离。