Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Encapsulated Nanodroplets for Enhanced Fluorescence Detection by Nano‐Extraction
Small ( IF 13.0 ) Pub Date : 2020-10-26 , DOI: 10.1002/smll.202004162 Miaosi Li 1, 2 , Rong Cao 3 , Brendan Dyett 4 , Xuehua Zhang 5
Small ( IF 13.0 ) Pub Date : 2020-10-26 , DOI: 10.1002/smll.202004162 Miaosi Li 1, 2 , Rong Cao 3 , Brendan Dyett 4 , Xuehua Zhang 5
Affiliation
|
Enhancement of the detection signal of fluorescence microscopy in highly diluted solutions is of great importance in chemical analysis, sensing, and bioassay applications. Surface nanodroplets with atto‐ to femto‐liter volumes are promising tools for sensitive online detection by integrating their extremely efficient nano‐extraction and optical advantages. In this paper, the development of novel basic units of nanodroplets‐in‐a‐microdroplet by simple solvent exchange is reported. The encapsulated nanodroplets are applied for ultrasensitive and online detection in fluorescence imaging. The biphasic nature of the droplet composite enables simultaneous extraction and enrichment of both hydrophobic and hydrophilic compounds. Furthermore, the desirable lensing effect of the curved surface of the nanodroplets enhances the collection of light emitted from the fluorophore extracted in the droplets by ≈60‐fold, allowing sensitive and quantitative analysis of the fluorophore using fluorescence microscopy. The results highlight the potential of encapsulated nanodroplets as a simple and innovative method of signal enhancement in chemical analysis. By integrating selective concentration, extraction, and sensitive detection, the encapsulated nanodroplets reported here may have broad applications in many chemical and biological matrices.
中文翻译：
封装的纳米液滴可通过纳米提取增强荧光检测
在高度稀释的溶液中，荧光显微镜检测信号的增强在化学分析，传感和生物测定应用中非常重要。通过整合其极其高效的纳米提取和光学优势，表面纳米滴的体积为10至毫微升，是用于灵敏在线检测的有前途的工具。本文报道了通过简单的溶剂交换开发新型微滴基本单元的方法。封装的纳米液滴可用于荧光成像中的超灵敏和在线检测。液滴复合物的两相性质使得能够同时提取和富集疏水和亲水化合物。此外，≈60倍，可以使用荧光显微镜对荧光团进行灵敏和定量的分析。结果突出了封装的纳米液滴作为化学分析中信号增强的简单创新方法的潜力。通过整合选择性浓缩，提取和灵敏检测，此处报道的封装纳米滴可能在许多化学和生物基质中具有广泛的应用。
更新日期：2020-11-27
中文翻译：
封装的纳米液滴可通过纳米提取增强荧光检测
在高度稀释的溶液中，荧光显微镜检测信号的增强在化学分析，传感和生物测定应用中非常重要。通过整合其极其高效的纳米提取和光学优势，表面纳米滴的体积为10至毫微升，是用于灵敏在线检测的有前途的工具。本文报道了通过简单的溶剂交换开发新型微滴基本单元的方法。封装的纳米液滴可用于荧光成像中的超灵敏和在线检测。液滴复合物的两相性质使得能够同时提取和富集疏水和亲水化合物。此外，≈60倍，可以使用荧光显微镜对荧光团进行灵敏和定量的分析。结果突出了封装的纳米液滴作为化学分析中信号增强的简单创新方法的潜力。通过整合选择性浓缩，提取和灵敏检测，此处报道的封装纳米滴可能在许多化学和生物基质中具有广泛的应用。
京公网安备 11010802027423号