Seeing is believing: Current methods to observe sperm acrosomal exocytosis in real time,Molecular Reproduction and Development

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Seeing is believing: Current methods to observe sperm acrosomal exocytosis in real time
Molecular Reproduction and Development ( IF 2.7 ) Pub Date : 2020-10-28 , DOI: 10.1002/mrd.23431
Paula A Balestrini ₁ , Martina Jabloñski ₁ , Liza J Schiavi-Ehrenhaus ₁ , Clara I Marín-Briggiler ₁ , Claudia Sánchez-Cárdenas ₂ , Alberto Darszon ₂ , Darío Krapf ₃ , Mariano G Buffone ₁

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Acrosomal exocytosis (AR) is a critical process that sperm need to undergo to fertilize an egg. The evaluation of the presence or absence of the acrosome is usually performed by using lectins or dyes in fixed cells. With this approach, it is neither possible to monitor the dynamic process of exocytosis and related molecular events while discriminating between live and dead cells, nor to evaluate the acrosomal status while sperm reside in the female reproductive tract. However, over the last two decades, several new methodologies have been used to assess the occurrence of AR in living cells allowing different groups to obtain information that was not possible in the past. These techniques have revolutionized the whole study of this process. This review summarizes current methods available to analyze AR in living cells as well as the important information that emerged from studies using these approaches.

中文翻译：

眼见为实：目前实时观察精子顶体胞吐的方法

顶体胞吐作用 (AR) 是精子使卵子受精所需的关键过程。通常通过在固定细胞中使用凝集素或染料来评估是否存在顶体。使用这种方法，既不可能在区分活细胞和死细胞的同时监测胞吐作用和相关分子事件的动态过程，也不可能在精子驻留在女性生殖道时评估顶体状态。然而，在过去的二十年里，已经使用了几种新方法来评估活细胞中 AR 的发生，从而使不同的群体能够获得过去不可能获得的信息。这些技术彻底改变了这一过程的整个研究。

更新日期：2020-12-30

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