This study was a single time‐point mapping of various immunostaining patterns revealed with the proliferating cell nuclear antigen (PCNA) PC10 antibody in spermatogonia at the immature pole of the testis of the Blue shark (Prionace glauca). Scattered in the stroma of the germinal ridge that demarcates the immature pole's outer boundary were nests of variously immunoreactive A‐spermatogonia, each flanked by a fusiform cell. Spermatocysts were assembled from niche‐derived stromal cells, displaced A‐progenitors, and their progeny, which showed one of two main immunostaining patterns (i.e., an uneven light brown/globular and homogeneous dark [hod] brown appearance). The testes of wild‐caught Prionace showed two conditions, namely, extensive multinucleate cell death (MNC) near the mitosis–meiosis transition or an early recovery phase from the latter showing vacuolated areas. Both the proportion of cysts with immature B^hod‐spermatogonia and the frequency of mitotic figures in such cysts in the early recovery testis condition were significantly higher than the comparable parameters in MNC testis condition. Moreover, the post‐MNC recovery phase revealed a decrease in the proportion of immature cysts with uneven light brown/globular‐like spermatogonia. The protracted spread of a cell cycle signal in an anatomically discrete, syncytially connected spermatogonial clone manifests as different PCNA immunoreactivities.

中文翻译：

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对成年野生蓝鲨幼鲨睾丸未成熟极处精原细胞增殖细胞核抗原的各种反复免疫染色模式的简单一次性绘制：与睾丸状态变化的相关性

这项研究是对各种免疫染色模式的单一时间点作图，这些模式是在蓝鲨 ( Prionace glauca )睾丸未成熟极处的精原细胞中发现的增殖细胞核抗原 (PCNA) PC10 抗体。在划分未成熟极外边界的生发脊的基质中散布着具有不同免疫反应性的 A 精原细胞的巢穴，每个巢穴两侧都有一个梭形细胞。精囊由来自生态位的基质细胞、置换的 A 祖细胞及其后代组装而成，它们显示出两种主要的免疫染色模式之一（即不均匀的浅棕色/球状和均匀的深 [hod] 棕色外观）。野生捕获的普里奥纳斯的睾丸显示两种情况，即有丝分裂 - 减数分裂过渡附近的广泛多核细胞死亡（MNC）或后者显示空泡区域的早期恢复阶段。在早期恢复睾丸条件下，具有未成熟 B ^hod精原细胞的包囊比例和此类包囊中的有丝分裂频率均显着高于 MNC 睾丸条件下的可比参数。此外，MNC 后恢复阶段显示，具有不均匀浅棕色/球状精原细胞的未成熟囊肿的比例减少。细胞周期信号在解剖学上离散、合胞体连接的精原细胞克隆中的长期传播表现为不同的 PCNA 免疫反应性。