Bone defect‐related surgical procedures are traumatic processes carrying potential inflammation and infection risks in the clinic, which are associated with prolonged antibiotic therapy that promotes bacterial antibiotic‐resistance. In the present study, Cu‐loaded brushite bone cements were designed, and the properties of the bone cements were evaluated. The setting time of the cement was prolonged from 12 to 50 min as the copper content increased. All cements were anti‐washout, and the injectable coefficient of the cements was approximately 88%. Scanning electron microscopy results revealed that the crystal grains grew larger and thicker as the copper content in the cement increased, and brushite was determined to be the dominant crystalline phase for all the cements. However, a small amount of newly formed calcium copper phosphate was observed in the cement. Simultaneously, band shifts were observed in the Fourier transform infrared spectroscopy results at a Cu content of 5%. Moreover, the addition of Cu improved the compressive strength of brushite cements, and all cements were degradable. Furthermore, the Cu‐loaded brushite bone cements performed well in inhibiting the growth and proliferation of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, and the diameter of the inhibition zone increased with increasing copper content. The study revealed that the Cu‐loaded brushite bone cements possessed good cellular affinity to mouse bone marrow stem cells when a lower dose of copper was added in vitro. These results support the great potential of injectable antibacterial brushite bone cement specifically for bone tissue defect‐related repair and regeneration.

中文翻译：

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具有良好抗菌活性和可操作性的载铜磷灰石骨水泥

骨缺损相关的外科手术是在临床上具有潜在炎症和感染风险的创伤性过程，这与促进细菌抗生素耐药性的长期抗生素治疗有关。在本研究中，设计了载铜的透钙磷石骨水泥，并评估了骨水泥的性能。随着铜含量的增加，水泥的凝结时间从 12 分钟延长到 50 分钟。所有水泥均为抗冲刷，水泥的可注射系数约为88%。扫描电镜结果显示，随着水泥中铜含量的增加，晶粒长大变粗，透钙钙石被确定为所有水泥的主要结晶相。然而，在水泥中观察到少量新形成的磷酸铜钙。同时，在 Cu 含量为 5% 时，在傅里叶变换红外光谱结果中观察到带移。此外，Cu的添加提高了透钙磷水泥的抗压强度，所有水泥均可降解。此外，载Cu的透钙磷石骨水泥在抑制骨水泥的生长和增殖方面表现良好。金黄色葡萄球菌、大肠埃希菌和铜绿假单胞菌，抑菌圈的直径随着铜含量的增加而增加。该研究表明，当体外添加较低剂量的铜时，载铜的透钙磷石骨水泥对小鼠骨髓干细胞具有良好的细胞亲和力。这些结果支持了可注射抗菌透钙石骨水泥的巨大潜力，专门用于骨组织缺损相关的修复和再生。