In this article, the enzyme kinetic activity of Mucor javanicus lipase (MJL) has investigated via hydrolysis of p‐nitrophenyl acetate (PNPA) in different pH environment, cationic surfactants, and inorganic salts. The influence of hydrophilicity has been studied in the presence of cationic cetyltrimethylammonium bromide (CTAB), cetyltriphenylphosphonium bromide (CTPB), and cetyldimethylethanolammonium bromide (CDMEAB) surfactants. The head group dimensions of surfactants show noteworthy contributions to enhance the catalytic activity of MJL, that is the CDMEAB surfactant, which is more efficient when compared with CTPB and CTAB. The increase in hydrophobicity of alkyltrimethylammonium bromide and alkyltriphenylphosphonium bromide in series dodecyl (C₁₂) < tetradecyl (C₁₄) < cetyl (C₁₆) leads to a marked decrease in the observed enzymatic superactivity, but an opposite trend appears in case of alkyldimethylammonium bromide. The activity of MJL for the hydrolysis of PNPA was higher in the presence of sodium chloride and potassium chloride anion due to specific interactions between the lipase surface and anions of the Hofmeister effect.

中文翻译：

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阳离子表面活性剂和无机盐对爪哇毛霉脂肪酶酶动力学活性的影响

在本文中，通过在不同pH环境下对硝基乙酸苯酯（PNPA），阳离子表面活性剂和无机盐的水解，研究了Mucor爪哇脂酶（MJL）的酶动力学活性。在阳离子十六烷基三甲基溴化铵（CTAB），十六烷基三苯基溴化phosph（CTPB）和十六烷基二甲基乙醇溴化铵（CDMEAB）表面活性剂的存在下，已经研究了亲水性的影响。表面活性剂的头基尺寸显示出对增强MJL（即CDMEAB表面活性剂）的催化活性的显着贡献，与CTPB和CTAB相比，其效率更高。十二烷基（C ₁₂）系列烷基三甲基溴化铵和烷基三苯基溴化bro的疏水性增加 <十四烷基（C ₁₄） <十六烷基（C ₁₆）导致所观察到的酶超活性显着降低，但是在烷基二甲基溴化铵的情况下出现相反的趋势。在氯化钠和氯化钾阴离子存在下，由于脂肪酶表面与霍夫迈斯特效应阴离子之间的特定相互作用，MJL水解PNPA的活性较高。