Stepwise Strategy for One‐Pot Synthesis of Single‐Stranded DNA Rings from Multiple Short Fragments,ChemBioChem

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Stepwise Strategy for One‐Pot Synthesis of Single‐Stranded DNA Rings from Multiple Short Fragments
ChemBioChem ( IF 2.6 ) Pub Date : 2020-10-30 , DOI: 10.1002/cbic.202000738
Zhe Sui ₁ , Ran An _{1,

2} , Makoto Komiyama ₁ , Xingguo Liang _{1,

2}

Affiliation

One at a time, please: Large, circular, single‐stranded DNAs (up to 452‐nt sizes) have been efficiently prepared from multiple linear, single‐stranded short DNA fragments by using T4 DNA ligase through sequential addition of different sets of splints in a one‐pot reaction. The lengths of splints and the order of splint addition were optimized to obtain a satisfying yield of c‐ssDNA products. This method is convenient and practical, and benefits the development of DNA nanotechnology and bioscience.

中文翻译：

从多个短片段一步法合成单链 DNA 环

请一次一个：使用 T4 DNA 连接酶通过连续添加不同的夹板，从多个线性单链短 DNA 片段有效地制备了大的环状单链 DNA（最大 452 nt 大小）在一锅反应中。对夹板的长度和夹板的添加顺序进行了优化，以获得令人满意的 c-ssDNA 产物产量。该方法方便实用，有利于DNA纳米技术和生物科学的发展。

更新日期：2020-10-30

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