In this study, various bacterial strains were isolated from date palm (Phoenix dactylifera L.) rhizosphere grown in an orchard with high soil salinity and tested for plant growth-promoting properties by producing microbial volatile compounds (mVOC). An Enterobacter cloacae SQU-2 (SQU-2) bacterial strain was identified, which enhanced the growth of Arabidopsis thaliana Columbia (Col-0) seedlings under both normal and salt stress conditions by producing mVOC. This rhizobacterium also possesses various growth promotion traits such as zinc and phosphorus solubilization, indole acetic acid (IAA), ammonia and siderophore production. Seeing these beneficial traits that the rhizobacterium commands, we sequenced the genome using next-generation sequencing methods to identify the potential growth promotion genes present in this rhizobacterium. Sequence annotation of the genome resulted in identifying various plant growth-promoting gene clusters, including siderophore production and the biosynthesis of mVOC such as 2,3-butanediol and acetoin. The presence of mVOC gene clusters in the genome provides genomic evidence on this bacterium's ability to produce mVOC, through which it enhanced the growth of Arabidopsis seedlings in vitro. The results obtained from this project may open an avenue toward further studies aimed to use this strain in agriculture and to determine the mechanism of plant-microbe interaction, which led to enhancing growth.

在这项研究中，从土壤盐分高的果园中生长的枣棕（Phoenix dactylifera L.）根际中分离出各种细菌菌株，并通过生产微生物挥发性化合物（mVOC）来测试其促进植物生长的特性。鉴定出一种阴沟肠杆菌SQU-2（SQU-2）细菌菌株，该菌株可增强拟南芥的生长。通过产生mVOC在正常和盐胁迫条件下的哥伦比亚（Col-0）幼苗。这种根瘤菌还具有各种促进生长的特性，例如锌和磷的增溶，吲哚乙酸（IAA），氨和铁载体的产生。看到了根瘤菌所具有的这些有益特性，我们使用下一代测序方法对基因组进行了测序，以鉴定存在于该根瘤菌中的潜在生长促进基因。基因组的序列注释导致鉴定各种促进植物生长的基因簇，包括铁载体的产生和mVOC的生物合成，例如2,3-丁二醇和乙酰丁香。基因组中mVOC基因簇的存在为该细菌产生mVOC的能力提供了基因组证据，通过它可以增强mVOC的生长。拟南芥幼苗的体外。从该项目获得的结果可能为进一步研究打下基础，该研究旨在将该菌株用于农业并确定植物与微生物的相互作用机制，从而促进生长。