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Assessment of A20 infectious laryngotracheitis vaccine take in meat chickens using swab and dust samples following mass vaccination in drinking water
Veterinary Microbiology ( IF 2.4 ) Pub Date : 2020-10-24 , DOI: 10.1016/j.vetmic.2020.108903
Awol M Assen 1 , Mark Stillman 2 , Sheridan Alfirevich 2 , Priscilla F Gerber 3 , Peter J Groves 4 , Stephen W Walkden-Brown 3
Affiliation  

Infectious laryngotracheitis, caused by the alphaherpesvirus infectious laryngotracheitis virus (ILTV), is an important disease of chickens. Partial control of this disease in meat chickens is commonly achieved by mass vaccination with live virus in drinking water. There is a need for a practical test to evaluate vaccination outcomes. For the Serva ILTV vaccine, quantitative real-time PCR (qPCR) enumeration of ILTV genome copies (GC) in flock level dust samples collected at 7–8 days post vaccination (dpv) can be used to differentiate flocks with poor and better vaccine take. This study aimed to validate this approach for A20, another widely used ILT vaccine in Australia. In four meat chicken flocks vaccinated with A20 in water using two different water stabilization times (20 or 40 min), swabs from the trachea and choanal cleft and dust samples were collected at 0, 7, 14 and 21 dpv. ILTV GC detection in swabs and dust was highest at 7 dpv and at this time ILTV GC load in dust was strongly and positively associated with vaccine take in individual birds assessed by swab samples. Choanal cleft swabs provided significantly fewer ILTV positive results than paired tracheal swab samples but the level of ILTV GC detected was similar. Water stabilization time had only minor effects on vaccination response in favour of the shorter time. Location of dust collection had no effect on viral load measured in dust samples. Dust samples collected at 0 and 7 dpv can be used to assess the vaccination status of flocks.



中文翻译:

在饮用水中大规模接种疫苗后,使用拭子和灰尘样品评估肉鸡的A20传染性喉气管炎疫苗接种情况

甲型疱疹病毒感染性喉气管炎病毒(ILTV)引起的传染性喉气管炎是鸡的重要疾病。通常通过在饮用水中大量接种活病毒来部分控制肉鸡中的这种疾病。需要进行实际测试以评估疫苗接种结果。对于Serva ILTV疫苗,可以在接种疫苗后7-8天(dpv)收集的鸡群粉尘样品中实时定量PCR(qPCR)枚举ILTV基因组拷贝(GC),以区分接种疫苗效果较好和效果较好的鸡群。这项研究旨在验证这种方法是否适用于澳大利亚另一种广泛使用的ILT疫苗A20。在使用两种不同的水稳定时间(20或40分钟)的情况下,在四只接种了A20的水中的肉鸡群中,分别从0、7、14和21 dpv采集气管和咽部裂口拭子,并收集尘土样品。拭子和灰尘中的ILTV GC检测最高,达到7 dpv,此时,灰尘中ILTV GC的含量与通过拭子样本评估的个体禽类的疫苗接种密切相关。与成对的气管拭子样本相比,裂婴儿拭子提供的ILTV阳性结果要少得多,但检测到的ILTV GC的水平相似。水分稳定时间对疫苗接种反应影响很小,有利于缩短时间。灰尘收集的位置对灰尘样品中测得的病毒载量没有影响。在0和7 dpv收集的粉尘样品可用于评估鸡群的疫苗接种状况。拭子和灰尘中的ILTV GC检测最高,达到7 dpv,此时,灰尘中ILTV GC的含量与通过拭子样本评估的个体禽类的疫苗接种密切相关。与成对的气管拭子样本相比,裂婴儿拭子提供的ILTV阳性结果要少得多,但检测到的ILTV GC的水平相似。水分稳定时间对疫苗接种反应影响很小,有利于缩短时间。灰尘收集的位置对灰尘样品中测得的病毒载量没有影响。在0和7 dpv收集的粉尘样品可用于评估鸡群的疫苗接种状况。拭子和尘埃中的ILTV GC检测最高,达到7 dpv,此时,尘埃中的ILTV GC负荷与通过拭子样本评估的个体禽类的疫苗接种量密切相关且呈正相关。与成对的气管拭子样本相比,裂婴儿拭子提供的ILTV阳性结果要少得多,但检测到的ILTV GC的水平相似。水分稳定时间对疫苗接种反应影响很小,有利于缩短时间。灰尘收集的位置对灰尘样品中测得的病毒载量没有影响。在0和7 dpv收集的粉尘样品可用于评估鸡群的疫苗接种状况。与成对的气管拭子样本相比,裂婴儿拭子提供的ILTV阳性结果要少得多,但检测到的ILTV GC的水平相似。水分稳定时间对疫苗接种反应影响很小,有利于缩短时间。灰尘收集的位置对灰尘样品中测得的病毒载量没有影响。在0和7 dpv收集的粉尘样品可用于评估鸡群的疫苗接种状况。与成对的气管拭子样本相比,裂婴儿拭子提供的ILTV阳性结果要少得多,但检测到的ILTV GC的水平相似。水分稳定时间对疫苗接种反应影响很小,有利于缩短时间。灰尘收集的位置对灰尘样品中测得的病毒载量没有影响。在0和7 dpv收集的粉尘样品可用于评估鸡群的疫苗接种状况。

更新日期:2020-11-09
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