This review covers preparative-scale chromatography for enantioseparations in both supercritical fluid and liquid chromatography published between 2016 and 2020. When pertinent, some fundamental publications from outside this time window are cited. The recent developments in instrumentation, novelties in method development and chiral stationary- and mobile phases are placed in context, while throughput, scale-up and sample issues are presented for supercritical fluid chromatography, and large-batch techniques together with computer-assisted method development, are described for liquid chromatography. Preparative-scale applications are reported for natural products, pharmaceutical compounds and pesticides, as well some other small molecules. Finally, a section is devoted to reports comparing supercritical fluid- and liquid chromatography and the strategies of various commercial users are briefly described. The goal of this review is to present current practices in the field of medium to large-scale chiral separations and offer indications as to how the best approach may be chosen for a given application.

这篇综述涵盖了2016年至2020年之间发表的超临界流体色谱和液相色谱中对映体分离的制备规模色谱。相关时，引用了此时间范围之外的一些基本出版物。本文介绍了仪器的最新进展，方法开发的新颖性以及手性固定相和流动相，同时介绍了超临界流体色谱法的通量，放大和样品问题以及大批量技术和计算机辅助方法的开发描述了液相色谱。据报道天然产物，药物化合物和农药以及其他一些小分子的制备规模应用。最后，本节专门介绍比较超临界流体色谱和液相色谱的报告，并简要介绍各种商业用户的策略。这篇综述的目的是介绍中至大规模手性分离领域的当前实践，并提供关于如何为给定应用选择最佳方法的指示。