Antibacterial and cytotoxic activities of Euphorbia balsamifera, fractions and pure compounds were evaluated. The cytotoxic assays for HCT116, HePG2 and MCF7 showed a significant IC₅₀: 54.7 and 76.2 µg/mL of non-polar fraction “n-hexane” against HCT116 and HePG2, respectively. Antibacterial results revealed that plant fractions exhibited significant potential against the tested pathogens than the total extract where n-butanol and ethyl acetate fractions showed significant antibacterial activity (P < 0.05) against tested bacterial strains. Isolation and structure determination of compounds from n-hexane and n-butanol fractions were performed. From n-hexane fraction, 29-nor-cycloartanol (1), lanost-8-en-3-ol (2a), cycloartanol (2b) and kampferol-3,4'-dimethyl ether (3) were isolated and structurally identified, along with 24 compounds were tentatively identified by GC–MS. From the polar n-butanol fraction, 4-O-β-D-glucopyranosyl-2-hydroxy-6-methoxyacetophenone (4), 4-O-α-L-rhamnosyl-(1 → 6)-β-D-glucopyranosyl-2-hydroxy-6methoxy-acetophenone (5), quercetin-3-O-glucopyranoside (6) and isoorientin (7) were assigned. Structures of the obtained compounds were determined by nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry. Except compounds 1 and 5, all reported compounds announced antibacterial efficiency. Compound 2 showed selectively the highest activity against Enterococcus faecalis (22 ± 0.13 mm), meanwhile 4-O-β-D-glucopyranosyl-2-hydroxy-6-methoxyacetophenone (4) showed broadly the highest antibacterial activity with MIC of 1.15–1.88 mg/mL against the test Gram-positive and Gram-negative bacteria. Cytotoxic assays indicated that kampferol-3,4'-dimethyl ether (3) exhibited the highest activity with matching IC₅₀ values to doxorubicin; 111.46, 42.67 and 44.90 µM against HCT116, HePG2 and MCF7, respectively, however, it is toxic on retina normal cell line RPE1.

评估了大戟，部分和纯化合物的抗菌和细胞毒性活性 。对于HCT116，HepG2和MCF-7的细胞毒性测定法表现出显著IC ₅₀：54.7和76.2微克/毫升的非极性级分“的Ñ正己烷”对HCT116和HepG2，分别。抗菌结果表明，与总提取物相比，植物级分对被测病原体表现出显着的潜力，而正丁醇和乙酸乙酯馏分 对被测细菌菌株表现出显着的抗菌活性（P <0.05）。从化合物的分离与结构确定Ñ己烷和Ñ进行丁醇馏分。从Ñ己烷馏分，29去甲cycloartanol（1）， lanost -8-烯-3-醇（2A）， cycloartanol （2B）和山柰酚-3,4'-二甲醚（3进行分离和结构鉴定），通过GC-MS初步鉴定了24种化合物。从极性正丁醇级分中，4- O -β -D-吡喃葡萄糖基-2-羟基-6-甲氧基苯乙酮（4）， 4- O -α-L-鼠李糖基-（1→6）-β-D-吡喃葡萄糖基-2-羟基-6甲氧基苯乙酮（5），槲皮素3- O-吡喃葡萄糖苷（6）和异Orientinin（7）被分配。所获得的化合物的结构通过核磁共振（NMR）光谱法和质谱法确定。除化合物1和5外，所有报道的化合物均具有抗菌作用。化合物2对粪肠球菌有选择性地表现出最高的活性（22±0.13 mm），而4- O -β-D-吡喃葡萄糖基-2-羟基-6-甲氧基苯乙酮（4）则表现出最高的抗菌活性，MIC为1.15–1.88相对于测试的革兰氏阳性和革兰氏阴性细菌的mg / mL。细胞毒性试验表明，坎普福尔3,4'-二甲醚（3）表现出最高的活性，并具有匹配的IC ₅₀阿霉素的价值；对HCT116，HePG2和MCF7分别为111.46、42.67和44.90 µM，但它对视网膜正常细胞RPE1有毒。