Deoxynivalenol (DON) is Fusarium mycotoxin that is frequently found in many cereal-based foods, and its ingestion has a deleterious impact on human health. In this investigation, we studied the mechanism of DON-induced neurotoxicity and followed by cytoprotective efficacy of quercetin (QUE) in contradiction of DON-induced neurotoxicity through assessing the oxidative stress and apoptotic demise in the human neuronal model, i.e. SH-SY5Y cells. DON diminished the proliferation of cells in the manner of dose and time-dependent as revealed by cell viability investigations, i.e. MTT and lactate dehydrogenase assays. Additional studies, such as intracellular reactive oxygen species (ROS), lipid peroxidation (LPO), mitochondrial membrane potential (MMP), DNA damage, cell cycle, and neuronal biomarkers (amino acid decarboxylase, tyrosine hydroxylase, and brain-derived neurotrophic factor) demonstrated that DON induces apoptotic demise in neuronal cells through oxidative stress intermediaries. On another hand, pre-treatment of neuronal cells with 1 mM of quercetin (QUE) showed decent viability upon exposure to 100 µM of DON. In detailed studies demonstrated that QUE (1 mM) pre-treated cells show strong attenuation efficiency against DON-induced ROS generation, LPO, MMP loss, DNA impairment, cell cycle arrest, and down-regulation of neuronal biomarkers. The consequences of the investigation concluded that QUE mitigates the DON-induced stress viz., decreased ROS production and LPO generation, upholding MMP and DNA integrity and regulation of neuronal biomarker gene expression in SH-SY5Y cells.

脱氧雪腐烯（DON）是镰刀菌霉菌毒素是许多谷物食品中经常发现的霉菌毒素，其摄入会对人体健康产生有害影响。在这项研究中，我们通过评估人类神经元模型（即SH-SY5Y细胞）的氧化应激和凋亡死亡，研究了DON诱导神经毒性的机制，然后研究了槲皮素（QUE）在DON诱导神经毒性中的细胞保护作用。DON以剂量和时间依赖性的方式减少了细胞的增殖，这是通过细胞活力研究（即MTT和乳酸脱氢酶测定）揭示的。其他研究，例如细胞内活性氧（ROS），脂质过氧化（LPO），线粒体膜电位（MMP），DNA损伤，细胞周期和神经元生物标志物（氨基酸脱羧酶，酪氨酸羟化酶，和脑源性神经营养因子）证明DON通过氧化应激介质在神经元细胞中诱导凋亡死亡。另一方面，用1 mM槲皮素（QUE）预处理神经元细胞在暴露于100 µM DON时显示出良好的生存能力。详细研究表明，QUE（1 mM）预处理的细胞对DON诱导的ROS生成，LPO，MMP丢失，DNA损伤，细胞周期停滞以及神经元生物标志物的下调具有很强的衰减作用。调查的结果得出结论，QUE减轻了DON引起的压力。详细研究表明，QUE（1 mM）预处理的细胞对DON诱导的ROS生成，LPO，MMP丢失，DNA损伤，细胞周期停滞以及神经元生物标志物的下调具有很强的衰减作用。调查的结果得出结论，QUE减轻了DON引起的压力。详细研究表明，QUE（1 mM）预处理的细胞对DON诱导的ROS生成，LPO，MMP丢失，DNA损伤，细胞周期停滞以及神经元生物标志物的下调具有很强的衰减作用。调查的结果得出结论，QUE减轻了DON引起的压力。，降低ROS产生和LPO生成，维持MMP和DNA完整性以及调节SH-SY5Y细胞中神经元生物标记基因表达的调控。