In the present study, four Lactobacillus strains from the cheese were analyzed for its probiotic potential against enteropathogenic bacteria. The probiotic properties of the selected strains were also analyzed and the selected bacterial strains showed high tolerance in bile salts and organic acid. The strain L. plantarum LP049 showed maximum survival rate (92 ± 4.2% and 93.3 ± 2%) after 3 h of treatment at 0.25% (w/v) bile salts and 0.25% (w/v) organic acid concentrations. The ability of the Lactobacillus strains to adhere to human epithelial cells (HT-29 cell lines) was evaluated and L. plantarum LP049 showed maximum adhesion property (19.2 ± 1.1%) than other tested strains. The Lactobacillus strains produced lactic acid at various concentrations. Compared with other strains, maximum level of lactic acid (3.1 g/L), hydrogen peroxide (4.31 mM) and bacteriocin (31 AU/mg) was detected in LB049. The inhibitory activity of culture supernatant against various bacterial pathogens was observed. The zone of inhibition ranged between 6 ± 2 mm and 23 ± 2 mm. The cell free extract showed activity against, Escherichia coli (ATCC 10536), Salmonella enteritidis (ATCC 13076), Shigella flexneri (ATCC 29903), and Enterococcus faecium (ATCC 8459). Consequently, L. plantarum LP049 may be considered as a potential candidate for the production of novel bioactive metabolites for therapeutic and bio-protective applications.

在本研究中，分析了奶酪中的四种乳酸杆菌菌株对肠道致病菌的益生菌潜力。还分析了所选菌株的益生菌性质，并且所选细菌菌株在胆盐和有机酸中显示出高耐受性。在0.25％（w / v）胆汁盐和0.25％（w / v）的有机酸浓度下处理3小时后，植物乳杆菌LP049菌株显示出最大存活率（92±4.2％和93.3±2％）。评估了乳杆菌菌株粘附于人上皮细胞（HT-29细胞系）的能力，并鉴定了植物乳杆菌LP049显示出比其他测试菌株更高的附着力（19.2±1.1％）。乳酸杆菌菌株产生各种浓度的乳酸。与其他菌株相比，在LB049中检测到最大水平的乳酸（3.1 g / L），过氧化氢（4.31 mM）和细菌素（31 AU / mg）。观察到培养物上清液对各种细菌病原体的抑制活性。抑制范围为6±2 mm至23±2 mm。无细胞提取物表现出抗大肠杆菌（ATCC 10536），肠炎沙门氏菌（ATCC 13076），弗氏志贺氏菌（ATCC 29903）和粪肠球菌（ATCC 8459）的活性。因此，植物乳杆菌 LP049可被认为是生产用于治疗和生物保护应用的新型生物活性代谢物的潜在候选者。